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首页> 外文期刊>Cytokine >Inflammatory and bone-related genes are modulated by aging in human periodontal ligament cells.
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Inflammatory and bone-related genes are modulated by aging in human periodontal ligament cells.

机译：炎症和骨骼相关基因受人牙周膜细胞老化的影响。

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Periodontal ligament cells (PDLC) play a major role in periodontal tissues homeostasis and destruction. Most age-associated diseases seem to be closely related to an underlying chronic inflammatory state. Thus, the present study aimed at evaluating in PDLC the effect of aging on the basal levels of inflammatory and bone-related genes. Primary PDLC cultures were obtained from subjects aged 15-20 years (control- n=5), and subjects aged more than 60 years (test- n=5). Proliferation, cell viability and total secreted protein assays were performed, and mRNA levels were quantitatively assessed for interleukin (IL)-1beta, IL-4, IL-6 and IL-8, and for receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoprotegerin (OPG) by real time PCR. Data analysis demonstrated that aging negatively influenced cell proliferation, whereas cell viability and total secreted protein were not affected (p>0.05). Gene expression analysis showed that mRNA levels for RANKL and IL-8 were not affected by aging (p>0.05) whereas, mRNA levels for IL-4 was significantly lower in aged cells (p<0.05) and OPG, IL-1beta and IL-6 mRNA levels were higher (p<0.05). Data analysis suggests that aging decreased the ability of PDLC to proliferate and modulated the expression of important inflammatory and bone-related genes in periodontal ligament cells, favoring a proinflammatory and an antiresorptive profile.
机译：牙周膜细胞（PDLC）在牙周组织的稳态和破坏中起主要作用。大多数与年龄有关的疾病似乎与潜在的慢性炎症状态密切相关。因此，本研究旨在评估PDLC中衰老对炎症和骨骼相关基因基础水平的影响。从15-20岁的受试者（对照组-n = 5）和60岁以上的受试者（试验-n = 5）获得了原代PDLC培养物。进行增殖，细胞活力和总分泌蛋白测定，并定量评估白细胞介素（IL）-1beta，IL-4，IL-6和IL-8的mRNA水平，以及核因子-κB配体的受体激活剂（RANKL） ）和实时PCR检测骨保护素（OPG）。数据分析表明衰老对细胞增殖有负面影响，而细胞活力和总分泌蛋白则不受影响（p> 0.05）。基因表达分析表明，RANKL和IL-8的mRNA水平不受衰老的影响（p> 0.05），而衰老细胞中IL-4的mRNA水平则显着降低（p <0.05），OPG，IL-1beta和IL -6 mRNA水平较高（p <0.05）。数据分析表明，衰老降低了PDLC增殖并调节牙周膜细胞中重要的炎症和骨骼相关基因表达的能力，有利于促炎和抗吸收。

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