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首页> 外文期刊>British Poultry Science >Evaluation of cockerel spermatozoa viability and motility by a novel enzyme based cell viability assay
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Evaluation of cockerel spermatozoa viability and motility by a novel enzyme based cell viability assay

机译：基于新型酶的细胞活力测定评价Cockerel精子的活力和运动性

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1. The results of spermatozoa assessment by the WST-8 (2-[2-methoxy-4-nitrophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H-tetrazolium, monosodium salt) assay, flow cytometry (FC) or computer-assisted sperm analysis (CASA) were compared.2. Different live/killed ratios of cockerel semen were serially diluted to 120, 60, and 30x10(6) cells/ml, and each sample was analysed by (1) WST-8 assay at 0, 10, 20, 30, 40, 50, 60min, (2) viability with FC, and (3) motility with CASA.3. The WST-8 reduction rate was closely correlated with spermatozoa viability and motility. The optimal semen concentration for the WST-8 assay was 120x10(6) cells/ml, and the standard curves for spermatozoa viability and motility predictions, respectively, were y(viability60)=162.8x + 104.96 (R-2=0.9594) after 60min of incubation and y(motility40)=225.09x + 96.299 (R-2=0.8475) after 40min of incubation.4. It was concluded that the WST-8 assay is useful for the practical evaluation of cockerel spermatozoa viability and motility. Compared to FC and CASA, the WST-8 assay does not require expensive and complex instrumentation in the lab. Furthermore, one well of the WST-8 reaction can be used to predict spermatozoa viability and motility at the same time, which all lead it to be efficient and economical for semen quality assessment.
机译：1. WST-8（2- [2-甲氧基-4-硝基苯基] -3- [4-硝基苯基] -5- [2,4-二硫苯基] -2H-四唑，单钠盐的精子评估结果测定，流式细胞术（Fc）或计算机辅助精子分析（Casa）进行比较。将公鸡精液的不同的活/杀死的比率连续稀释至120,60和30x10（6）个细胞/ ml，并通过（1）在0,10,20,30,40,50的（1）威斯-8测定分析每个样品，60分钟，（2）与Fc的活力，和（3）Casa3的动力。 WST-8减少率与精子的活力和运动密切相关。 WST-8测定的最佳精液浓度为120×10（6）个细胞/ mL，分别为y（活性60）= 162.8x + 104.96（R-2 = 0.9594）孵育40分钟后，温育60分钟和Y（胰岛素40）= 225.09x + 96.299（R-2 = 0.8475）。得出结论是，WST-8测定对于公鸡精子的实际评估是有用的。与FC和CASA相比，WST-8测定不需要在实验室中昂贵而复杂的仪器。此外，WST-8反应的一个孔可用于预测同一时间的精子活力和运动，这都导致精液质量评估的有效和经济。

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