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Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling

机译：使用荧光脂质有助于脂质信号引导的原理的主动学习

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摘要

The concepts of phospholipase activity is often taught in undergraduate biology and biochemistry classes and reinforced in laboratory exercises. However, very rarely does the design of these exercises allow students to directly gain experience in the use of modern instruments such as digital imaging systems and fluorescence spectrophotometers. The laboratory exercise described here involves the use of fluorescent lipids to evaluate phospholipase activity. Students use thin layer chromatography (TLC) to understand how lipids change under different conditions (i.e. abiotic and biotic stress). They explore strategies to separate, visualize and quantify lipids by TLC, digital imaging, and fluorometry. They also have increased opportunities for hands-on practise with experimental design, liposome sample preparation, and implementation of instrumentation commonly used by experienced researchers; all while learning and applying fundamental concepts about lipids. (c) 2018 International Union of Biochemistry and Molecular Biology, 47(1):100-105, 2018.
机译：磷脂酶活性的概念通常在本科生物学和生物化学课程中教导，并在实验室练习中加强。然而，这些练习的设计很少允许学生直接在使用现代仪器等数字成像系统和荧光分光光度计等方面获得经验。这里描述的实验室运动涉及使用荧光脂质来评估磷脂酶活性。学生使用薄层色谱（TLC）来了解脂质在不同条件下如何变化（即非生物和生物应激）。他们探索通过TLC，数字成像和荧光测定分离，可视化和量化脂质的策略。他们还增加了实验设计，脂质体样品制备和经验丰富的研究人员常用的仪器的实施机会;一切都在学习和应用关于脂质的基本概念。 （c）2018年国际生物化学和分子生物联盟，47（1）：100-105，2018。

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