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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Aptamer based photoelectrochemical cytosensor with layer-by-layer assembly of CdSe semiconductor nanoparticles as photoelectrochemically active species
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Aptamer based photoelectrochemical cytosensor with layer-by-layer assembly of CdSe semiconductor nanoparticles as photoelectrochemically active species

机译：基于适体的光电化学细胞传感器，CdSe半导体纳米粒子的层层组装作为光电化学活性物质

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摘要

A label-free photoelectrochemical cytosensor for highly sensitive and specific detection of Ramos cell was developed based on photoactive films. The films were fabricated by a layer-by-layer (LBL) assembly of positively charged poly(dimethyldiallylammonium chloride) (PDDA) and negatively charged CdSe semiconductor nanoparticles (NPs) capped with mercaptoacetic acid. The resulting modified electrodes were tested as sensors for Ramos cell through the recognition of DNA aptamer which was covalently bound to the electrode using the classic coupling reactions between -COOH groups on the surfaces of CdSe NPs and -NH2 groups of the aptamer. The newly developed cytosensor exhibited excellent sensitivity and selectivity. The linear range was from 160 to 1600cells/mL and the detection limit was 84cells/mL.
机译：基于光敏膜，开发了一种用于高灵敏度和特异性检测Ramos细胞的无标记光电化学细胞传感器。通过带正电荷的聚二甲基二烯丙基氯化铵（PDDA）和带巯基乙酸封端的带负电荷的CdSe半导体纳米颗粒（NP）的逐层（LBL）组装来制造薄膜。通过识别CdSe NPs表面上的-COOH基团与适体的-NH2基团之间的经典偶联反应，通过识别与该电极共价结合的DNA适体，测试所得修饰电极作为Ramos细胞的传感器。新开发的细胞传感器具有出色的灵敏度和选择性。线性范围为160至1600个细胞/ mL，检出限为84个细胞/ mL。

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