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首页> 外文期刊>Cytokine >Effect of Astragalus polysaccharides on expression of TNF-α, IL-1β and NFATc4 in a rat model of experimental colitis
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Effect of Astragalus polysaccharides on expression of TNF-α, IL-1β and NFATc4 in a rat model of experimental colitis

机译：黄芪多糖对实验性结肠炎大鼠模型TNF-α，IL-1β和NFATc4表达的影响

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Aim: Astragalus membranaceus is a Chinese medicinal herb and has been shown to improve hapten-induced experimental colitis. One of its major components is polysaccharides. We investigated the effect of Astragalus polysaccharides (APS) on expression of TNF-α, IL-1β and NFATc4 in a rat model of experimental colitis. Methods: The experimental colitis model was induced by TNBS. Forty five rats were divided into five groups (n=. 9): Normal control group, receiving ethanol vehicle with no TNBS during induction and IP saline injection during treatment; TNBS colitis model group (TNBS. +. IP saline), receiving only IP saline vehicle treatment; APS low dose group (TNBS. +. L-APS), receiving APS 100. mg/kg; APS high dose group (TNBS. +. H-APS), receiving APS 200. mg/kg; and positive control group (TNBS. +. Dexm), receiving dexamethasone 0.3. mg/kg. The clinical features, macroscopic and microscopic scores were assessed. The expressions of TNF-α, IL-1β and NFATc4 were measured by real-time PCR and ELISA assays. Results: Compared to normal control rats, TNBS. +. IP saline had significant weight loss, increased macroscopic and microscopic scores, higher disease activity index (DAI) up-regulation of TNF-α, IL-1β and NFATc4 mRNA expression and up-regulation of TNF-α and IL-1β protein expression. Compared to TNBS. +. IP saline, treatment with APS or dexamethasone significantly reduced DAI, partially but significantly prevented TNBS colitis-induced weight loss and improved both macroscopic and microscopic scores; high dose APS or dexamethasone significantly down-regulated TNF-α and IL-1β expressions (both mRNA and protein) and up-regulated NFATc4 mRNA and protein expression. The effect of high dose APS and dexamethasone is comparable. Conclusions: APS significantly improved experimental TNBS-induced colitis in rats through regulation of TNF-α, IL-1β and NFATc4 expression.
机译：目的：黄芪是一种中草药，已被证明可以改善半抗原诱发的实验性结肠炎。其主要成分之一是多糖。我们研究了黄芪多糖（APS）对实验性结肠炎大鼠模型中TNF-α，IL-1β和NFATc4表达的影响。方法：用TNBS建立实验性结肠炎模型。将45只大鼠分为5组（n = 9）：正常对照组，在诱导过程中接受无TNBS的乙醇载体，在治疗过程中接受IP盐水注射。 TNBS结肠炎模型组（TNBS。+。IP生理盐水），仅接受IP生理盐水媒介治疗; APS低剂量组（TNBS。+。L-APS），接受APS 100. mg / kg; APS高剂量组（TNBS。+。H-APS），接受APS 200. mg / kg;和阳性对照组（TNBS。+。Dexm），接受地塞米松0.3。毫克/千克评估临床特征，宏观和微观得分。实时定量PCR和ELISA法检测TNF-α，IL-1β和NFATc4的表达。结果：与正常对照组相比，TNBS。 +。 IP盐水具有明显的体重减轻，宏观和微观评分增加，疾病活动指数（DAI）上调TNF-α，IL-1β和NFATc4 mRNA表达以及上调TNF-α和IL-1β蛋白表达。与TNBS相比。 +。 IP盐水，APS或地塞米松治疗可显着降低DAI，部分但可预防TNBS结肠炎引起的体重减轻，并改善宏观和微观评分。高剂量的APS或地塞米松显着下调TNF-α和IL-1β表达（mRNA和蛋白），并上调NFATc4 mRNA和蛋白表达。大剂量APS和地塞米松的效果相当。结论：APS通过调节TNF-α，IL-1β和NFATc4表达，显着改善了TNBS所致大鼠结肠炎。

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