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首页> 外文期刊>Cytokine >Orphan receptor GPR15/BOB is up-regulated in rheumatoid arthritis
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Orphan receptor GPR15/BOB is up-regulated in rheumatoid arthritis

机译：类风湿关节炎中的孤儿受体GPR15 / BOB上调

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Chemokine receptors on leukocytes mediate the recruitment and accumulation of these cells within affected joints in chronic inflammatory diseases such as rheumatoid arthritis (RA). Identification of involved receptors offers potential for development of therapeutic interventions. The objective of this study was to investigate the expression of orphan receptor GPR15/BOB in the synovium of RA and non-RA patients and in peripheral blood of RA patients and healthy donors. GPR15/BOB protein and messenger RNA expression were examined in RA and non-RA synovium by immunofluorescence and reverse-transcription polymerase chain reaction (RT-PCR) respectively. GPR15/BOB expression on peripheral blood leukocytes was analysed by flow cytometry and GPR15/ BOB messenger RNA was examined in peripheral blood monocytes by RT-PCR. GPR15/BOB protein was observed in CD68+ and CD14+ macrophages in synovia, with greater expression in RA synovia. GPR15/BOB protein was expressed in all patient synovia whereas in non-RA synovia expression was low or absent. Similarly GPR15/BOB messenger RNA was detected in all RA and a minority of non-RA synovia. GPR15/BOB protein was expressed on peripheral blood leukocytes from RA and healthy individuals with increased expression by monocytes and neutrophils in RA. GPR15/BOB messenger RNA expression was confirmed in peripheral blood monocytes. In conclusion GPR15/BOB is expressed by macrophages in synovial tissue and on monocytes and neutrophils in peripheral blood, and expression is up-regulated in RA patients compared to non-RA controls. This orphan receptor on monocytes/macrophages and neutrophils may play a role in RA pathophysiology.
机译：在慢性炎症性疾病如类风湿关节炎（RA）中，白细胞上的趋化因子受体介导这些细胞在受影响关节内的募集和积累。参与受体的鉴定为治疗干预的发展提供了潜力。这项研究的目的是研究孤儿受体GPR15 / BOB在RA和非RA患者的滑膜以及RA患者和健康供体的外周血中的表达。通过免疫荧光和逆转录聚合酶链反应（RT-PCR）分别检测RA和非RA滑膜中GPR15 / BOB蛋白和信使RNA的表达。通过流式细胞术分析外周血白细胞上的GPR15 / BOB表达，并通过RT-PCR检查外周血单核细胞中的GPR15 / BOB信使RNA。在滑膜的CD68 +和CD14 +巨噬细胞中观察到GPR15 / BOB蛋白，在RA滑膜中表达更高。 GPR15 / BOB蛋白在所有患者滑膜中表达，而在非RA滑膜中表达低或不存在。同样，在所有RA和少数非RA滑膜中也检测到GPR15 / BOB信使RNA。 GPR15 / BOB蛋白在RA和健康个体的外周血白细胞上表达，RA中单核细胞和中性粒细胞的表达增加。在外周血单核细胞中证实了GPR15 / BOB信使RNA表达。总之，GPR15 / BOB在滑膜组织，外周血单核细胞和嗜中性粒细胞中由巨噬细胞表达，与非RA对照相比，RA患者的表达上调。单核细胞/巨噬细胞和中性粒细胞上的这种孤儿受体可能在RA病理生理中起作用。

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