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Interaction of Hsp90 with phospholipid model membranes

机译：HSP90与磷脂模型膜的相互作用

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摘要

Abstract Heat shock protein 90 (Hsp90) is an essential molecular chaperone with versatile functions in cell homeostatic control under both normal and stress conditions. Hsp90 has been found to be expressed on the cell surface, but the mechanism of Hsp90 association to the membrane remains obscure. In this study, the direct interaction of Hsp90 and phospholipid vesicles was characterized, and the role of Hsp90 on membrane physical state was explored. Using surface plasmon resonance (SPR), we observed a strong interaction between Hsp90 and different compositions of lipid. Hsp90 had a preference to bind with more unsaturated phospholipid species and the affinity was higher with negatively charged lipids than zwitterionic lipids. Increasing the mole fraction of cholesterol in the phospholipid led to a decrease of binding affinity to Hsp90. Circular dichroism (CD) spectroscopy of Hsp90 in PC membranes showed more α-helix structure than in aqueous buffer. The differential scanning calorimeter (DSC) and fluorescence polarization results showed Hsp90 could affect the transition temperature and fluidity of the bilayer. We postulate from these results that the association between Hsp90 and membranes may involve both electrostatic and hydrophobic force, and constitute a possible mechanism that modulates membrane lipid order during thermal fluctuations. Graphical abstract Display Omitted Highlights ? Hsp90 had varied binding affinity with different compositions of lipids. ? The α-helical structure of Hsp90 increased upon binding with lipid vesicles. ? Hsp90 could increase the phase transition temperature and lipid order of membranes.
机译：摘要热休克蛋白90（HSP90）是一种必不可少的分子伴侣，在正常和应力条件下具有细胞稳态控制的通用功能。已发现HSP90在细胞表面上表达，但HSP90与膜的机制仍然模糊。在该研究中，探讨了HSP90和磷脂囊泡的直接相互作用，并探讨了HSP90对膜物理状态的作用。使用表面等离子体共振（SPR），我们观察到HSP90与不同组成的脂质之间的强相互作用。 HSP90优选与更多不饱和磷脂物质结合，并且亲和力较高，带有带负电荷的脂质比两性离子脂质。增加磷脂中胆固醇的摩尔摩尔级分导致对Hsp90结合的降低。在PC膜中Hsp90的圆形二中间铋（CD）光谱显示比在水性缓冲液中更多的α-螺旋结构。差分扫描量热计（DSC）和荧光偏振结果显示HSP90可能影响双层的过渡温度和流动性。我们从这些结果假设HSP90和膜之间的关联可能涉及静电和疏水力，并构成在热波动期间调节膜脂质顺序的可能机制。图形抽象显示省略了亮点？ HSP90与不同的脂质组成具有不同的结合亲和力。还HSP90的α-螺旋结构在与脂质囊泡结合后增加。还HSP90可以增加膜的相转变温度和脂质顺序。

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  • 作者

    Muhan Zhang; Daoying Wang; Pengpeng Li; Chong Sun; Rong Xu; Zhiming Geng; Weimin Xu; Zhaoqi Dai;

  • 作者单位

    Institute of Agricultural Products Processing Jiangsu Academy of Agricultural Sciences;

    Institute of Agricultural Products Processing Jiangsu Academy of Agricultural Sciences;

    Institute of Agricultural Products Processing Jiangsu Academy of Agricultural Sciences;

    Institute of Agricultural Products Processing Jiangsu Academy of Agricultural Sciences;

    Institute of Agricultural Products Processing Jiangsu Academy of Agricultural Sciences;

    Institute of Agricultural Products Processing Jiangsu Academy of Agricultural Sciences;

    Institute of Agricultural Products Processing Jiangsu Academy of Agricultural Sciences;

    Institute of Agricultural Products Processing Jiangsu Academy of Agricultural Sciences;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物膜的结构和功能;
  • 关键词

    Hsp90; Lipid binding; Membrane; SPR;

    机译：HSP90;脂质结合;膜;SPR;

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