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首页> 外文期刊>Biochimica et biophysica acta. Biomembranes >Detailed characterization of Synechocystis PCC 6803 ferredoxin:NADP + oxidoreductase interaction with model membranes
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Detailed characterization of Synechocystis PCC 6803 ferredoxin:NADP + oxidoreductase interaction with model membranes

机译：SyneChocystis PCC 6803 Ferredoxin的详细表征：NADP +氧化还原酶与模型膜的相互作用

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Abstract Direct interaction of ferredoxin:NADP + oxidoreductase (FNR) with thylakoid membranes was postulated as a part of the cyclic electron flow mechanism. In vitro binding of FNR to digalactosyldiacylglycerol and monogalactosyldiacylglycerol membranes was also shown. In this paper we deal with the latter interaction in more detail describing the effect for two FNR forms of Synechocystis PCC 6803. The so-called short FNR (sFNR) is homologous to FNR from higher plant chloroplasts. The long FNR (lFNR) form contains an additional domain, responsible for the interaction with phycobilisomes. We compare the binding of both sFNR and lFNR forms to native and non-native lipids. We also include factors which could modulate this process: pH change, temperature change, presence of ferredoxin, NADP + and NADPH and heavy metals. For the lFNR, we also include phycobilisomes as a modulating factor. The membrane binding is generally faster at lower pH. The sFNR was binding faster than lFNR. Ferredoxin isoforms with higher midpoint potential, as well as NADPH and NADP + , weakened the binding. Charged lipids and high phosphate promoted the binding. Heavy metal ions decreased the rate of membrane binding only when FNR was preincubated with them before injection beneath the monolayer. FNR binding was limited to surface lipid groups and did not influence hydrophobic chain packing. Taken together, FNR interaction with lipids appears to be non-specific, with an electrostatic component. This suggests that the direct FNR interaction with lipids is most likely not a factor in directing electron transfer, but should be taken into account during in vitro studies. Graphical abstract Display Omitted Highlights ? In vitro binding of ferredoxin:NADP + oxidoreductase (FNR) to model membranes was examined. ? Several lipids, native and non-native to the FNR, were tested. ? Short and long isoforms of FNR from Synechocystis sp . were used as model FNRs. ? Non-lipid factors, which potentially modulate the binding process, were included in the study. ? FNR interaction with lipids is most likely not a factor in directing electron transfer.
机译：摘要Ferridoxin的直接相互作用：NADP +氧化还原酶（FNR）与囊体膜的一部分假设为循环电子流动机理。还显示了FNR对二alactosyldiacylGlycerol和单硫酰基二酰基甘油膜的体外结合。在本文中，我们更详细地处理后一种交互，描述了对SyneChocystis PCC 6803的两种FNR形式的效果。所谓的短FNR（SFNR）与来自高等植物叶绿体的FNR同源。 LONG FNR（LFNR）形式包含另外的域，负责与植物霉菌的相互作用。我们将SFNR和LFNR形式的结合与本地和非本地脂质进行比较。我们还包括可以调节该过程的因素：pH变化，温度变化，富勒氏蛋白，NADP +和NADPH和重金属的存在。对于LFNR，我们还将植物霉素作为调节因子。膜结合通常在较低的pH下更快。 SFNR比LFNR更快地结合。富勒森蛋白同种型具有较高的中点电位，以及NADPH和NADP +，削弱了结合。带电脂质和高磷酸盐促进了结合。当在单层后注射之前用它们预孵育FNR时，重金属离子仅降低膜结合速率。 FNR结合仅限于表面脂质基团并且不影响疏水性链填料。连同，与脂质的FNR相互作用似乎是非特异性的，具有静电组分。这表明与脂质的直接FNR相互作用很可能不是指导电子转移的因素，但在体外研究时应考虑。图形抽象显示省略了亮点？研究了富勒沙昔姻的体外结合：研究了对模型膜的NADP +氧化还原酶（FNR）。还测试了几种脂质，天然和非原生对FNR。还来自SyneChocystis SP的FNR短和长同种型。用作模型FNRS。还潜在地调节结合过程的非脂质因子被包括在研究中。还与脂质的FNR相互作用很可能不是引导电子转移的因素。

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