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首页> 外文期刊>Biochimica et biophysica acta. Biomembranes >Antifungal activity of Latarcin 1 derived cell-penetrating peptides against Fusarium solani
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Antifungal activity of Latarcin 1 derived cell-penetrating peptides against Fusarium solani

机译：拉丁林1衍生的细胞穿透肽对Fusarium solani的抗真菌活性

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Abstract Cell-penetrating peptides and antimicrobial peptides share physicochemical characteristics and mechanisms of interaction with biological membranes, hence, termed as membrane active peptides. The present study aims at evaluating AMP activity of CPPs. LDP-NLS and LDP are Latarcin 1 derived cell-penetrating peptides and in the current study we have evaluated antifungal and cell-penetrating properties of these CPPs in Fusarium solani . We observed that LDP-NLS and LDP exhibited excellent antifungal activity against the fungus. Cellular uptake experiments with LDP-NLS and LDP showed that LDP-NLS acted as a CPP but LDP uptake into fungal spores and hyphae was negligible. CPP and AMP activity of mutated version of LDP-NLS was also evaluated and it was observed that both the activities of the peptide were compromised, signifying the importance of arginines and lysines present in LDP-NLS for initial interaction of membrane active peptides with biological membranes. Dextrans and Propidium Iodide uptake studies revealed that the mode of entry of LDP-NLS into fungal hyphae is through pore formation. Also, both LDP-NLS and LDP showed no cytotoxicity when infiltered into leaf tissues. Overall, our results suggest that LDP-NLS and LDP are selectively cytotoxic to F . solani and can be a potent peptide based antifungal agents. Graphical abstract Display Omitted Highlights ? LDs exhibit antifungal activity against Fusarium solani ? LDP-NLS shows enhanced cell-penetrating activity and retain antifungal activity in F . solani ? LDP-NLS damages F . solani cell membrane by pore formation
机译：摘要细胞穿透肽和抗微生物肽与生物膜的含有物理化学特性及其相互作用机制，因此称为膜活性肽。本研究旨在评估CPP的AMP活动。 LDP-NLS和LDP是LaTarcin 1衍生的细胞穿透肽，并且在目前的研究中，我们在镰刀菌索兰尼中评估了这些CPP的抗真菌和细胞穿透性质。我们观察到LDP-NLS和LDP对真菌具有优异的抗真菌活性。具有LDP-NLS和LDP的细胞摄取实验表明，LDP-NLS作为CPP，但LDP吸收到真菌孢子和菌丝可忽略不计。还评估了LDP-NLS的突变版的CPP和AMP活性，观察到肽的活性受到损害，这意味着在LDP-NL中存在的精氨酸和赖氨酸的重要性，用于膜活性肽与生物膜的初始相互作用。葡聚糖和碘化丙啶吸收研究表明，LDP-NLS进入真菌菌丝的方式是通过孔形成。此外，LDP-NLS和LDP均在浸入叶组织时显示出细胞毒性。总体而言，我们的结果表明LDP-NLS和LDP选择性地对F的细胞毒性。索兰尼，可以是基于有效的肽的抗真菌剂。图形抽象显示省略了亮点？ LDS表现出对Fusarium solani的抗真菌活性？ LDP-NLS显示增强的细胞穿透活性，并在F中保留抗真菌活性。 Solani？ LDP-NLS损坏F.梭菌细胞膜通过孔形成

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