• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochimica et biophysica acta. Biomembranes >Ib-AMP4 insertion causes surface rearrangement in the phospholipid bilayer of biomembranes: Implications from quartz-crystal microbalance with dissipation
【24h】

Ib-AMP4 insertion causes surface rearrangement in the phospholipid bilayer of biomembranes: Implications from quartz-crystal microbalance with dissipation

机译：IB-AMP4插入导致Biomembranes的磷脂双层中的表面重排：从石英晶体微稳定与耗散的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Most antimicrobial peptides exert their rapid bactericidal activity through a unique mechanism of bacterial membrane disruption. However, the molecular events that underlie this mechanism remain partly unresolved. In this study, the frequency shift (Δ F ) obtained through quartz-crystal microbalance with dissipation (QCM–D) indicated that the initial binding of Ib-AMP4 within the lipid membrane started at a critical Ib-AMP4 concentration that exceeded 100μg/ml. Circular dichroism measurements provided evidence that Ib-AMP4 occurs in a β-sheet configuration which is adapted for insertion into the lipid membrane. Monolayer experiments and the value of dissipation alteration (Δ D ) obtained through QCM–D showed that the pressure increased within the phospholipid bilayer upon peptide insertion, and the increase in pressure subsequently forced the bilayer to wrinkle and form pores. However, D continued to increase, indicating that the membrane surface underwent a dramatic morphological transition: the membrane surface likely became porous and uneven as Ib-AMP4 projected from the external surface of the lipid bilayer. Intensive peptide insertion, however, soon plateaued 1min after the addition of Ib-AMP4. This behaviour corresponded with the results of bactericidal kinetics and liposome leakage assays. A sudden decrease in D accompanied by a negligible decrease in F occurred after replacing the Ib-AMP4 solution with HEPES buffer. This result implied that the bilayer surface rearranged and that poration and wrinkling decreased without further peptide insertion. Transmission electron microscopy results indicated that pore formation occurred during Ib-AMP4 insertion but eventually subsided. Therefore, the mode of action of AMP in bacterial membranes could be elucidated through QCM–D. Graphical abstract Display Omitted Highlights ? Ib-AMP4 inserted into lipid bilayer after adopting a beta-sheet conformation ? Ib-AMP4 ruptured lipid vesicles through a non-lytic way. ? Ib-AMP4 inserted into the lipid bilayer and forced the bilayer undergoing a rearrangement
机译：摘要大多数抗菌肽通过独特的细菌膜破坏机制施加快速杀菌活性。然而，利于该机制的分子事件仍未分解。在该研究中，通过耗散（QCM-D）通过石英晶微相获得的频移（δf）表明，脂质膜内的IB-AMP4内的初始结合以超过100μg/ mL的关键IB-AMP4浓度开始。圆形二色性测量规定了证据表明IB-AMP4在β-片状构型中发生，其适于将其插入脂膜中。通过QCM-D获得的单层实验和耗散改变（Δd）的值表明，磷脂双层在肽插入时增加了压力，随后压力的增加迫使双层皱纹和形成孔。然而，D继续增加，表明膜表面经历了显着的形态转变：膜表面可能从脂质双层的外表面突出的IB-AMP4突出。然而，在加入IB-AMP4后，密集的肽插入即将推动1min。这种行为与杀菌动力学和脂质体渗漏测定的结果相对应。用HEPES缓冲液更换IB-AMP4溶液后，D突然减少了F的忽略不计的F.该结果暗示双层表面重新排列，并且在不进一步的肽插入的情况下降低了合影和皱纹。透射电子显微镜结果表明IB-AMP4插入期间发生孔隙形成，但最终会消退。因此，可以通过QCM-D阐明细菌膜中AMP的作用方式。图形抽象显示省略了亮点？在采用β-片状构象后插入脂质双层的IB-AMP4？ IB-AMP4通过非裂清方式破坏了脂质囊泡。还IB-AMP4插入脂质双层并迫使双层接受重排

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号