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首页> 外文期刊>Biochimica et biophysica acta. Biomembranes >Assessment of synergistic antibacterial activity of combined biosurfactants revealed by bacterial cell envelop damage
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Assessment of synergistic antibacterial activity of combined biosurfactants revealed by bacterial cell envelop damage

机译：细菌细胞包围损伤揭示的合并生物表面活性剂的协同抗菌活性的评估

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Abstract Besides potential surface activity and some beneficial physical properties, biosurfactants express antibacterial activity. Bacterial cell membrane disrupting ability of rhamnolipid produced by Pseudomonas aeruginosa C2 and a lipopeptide type biosurfactant, BS15 produced by Bacillus stratosphericus A15 was examined against Staphylococcus aureus ATCC 25923 and Escherichia coli K8813. Broth dilution technique was followed to examine minimum inhibitory concentration (MIC) of both the biosurfactants. The combined effect of rhamnolipid and BS15 against S . aureus and E . coli showed synergistic activity by expressing fractional inhibitory concentration (FIC) index of 0.43 and 0.5. Survival curve of both the bacteria showed bactericidal activity after treating with biosurfactants at their MIC obtained from FIC index study as it killed >90% of initial population. The lesser value of MIC than minimum bactericidal concentration (MBC) of the biosurfactants also supported their bactericidal activity against both the bacteria. Membrane permeability against both the bacteria was supported by amplifying protein release, increasing of cell surface hydrophobicity, withholding capacity of crystal violet dye and leakage of intracellular materials. Finally cell membrane disruption was confirmed by scanning electron microscopy (SEM). All these experiments expressed synergism and effective bactericidal activity of the combination of rhamnolipid and BS15 by enhancing the bacterial cell membrane permeability. Such effect of the combination of rhamnolipid and BS15 could make them promising alternatives to traditional antibiotic in near future. Graphical abstract Display Omitted Highlights ? Combination of rhamnolipid and BS15 expresses synergism against S . aureus and E . coli . ? Combination of test biosurfactants shows bactericidal activity. ? The antibacterial activity is expressed by bacterial membrane disruption.
机译：摘要除了潜在的表面活性和一些有益的物理性质之外，生物表面活性剂表达抗菌活性。通过假单胞菌铜绿假单胞菌和脂肽型生物活性剂产生的rhamnolipid的细菌细胞膜破坏能力，BS15通过芽孢杆菌ATCC 25923和大肠杆菌K8813检测由芽孢杆菌划分A15产生的BS15。遵循肉汤稀释技术，检查两种生物表面活性剂的最小抑制浓度（MIC）。 rhamnolipid和BS15对S的综合作用。金黄色葡萄球菌和e。 Coli通过表达0.43和0.5的分数抑制浓度（FIC）指数显示协同活性。在从FIC指数研究中获得的生物表面活性剂处理后，细菌的存活曲线显示出在其麦克风中获得的生物表面活性剂，因为它被杀死> 90％的初始群体。 MIC的较小值比生物件的最小杀菌浓度（MBC）也支持对细菌的杀菌活性。通过扩增蛋白质释放，增加细胞表面疏水性，尤其紫色染料泄漏的膜渗透，增加对细菌的膜渗透性。最后通过扫描电子显微镜（SEM）来确认细胞膜破坏。所有这些实验通过增强细菌细胞膜渗透性表达了rhamnolipid和BS15的组合的协同作用和有效的杀菌活性。 rhamnolipid和BS15组合的这种效果可以在不久的将来使其在传统抗生素中具有前景的替代品。图形抽象显示省略了亮点？ rhamnolipid和bs15的组合表达了对s的协同作用。金黄色葡萄球菌和e。大肠杆菌。还试验生物表面活性剂的组合显示杀菌活性。还抗菌活性由细菌膜破坏表示。

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