...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioanalysis >Analytical Quality by Design-based development and validation of ultra pressure liquid chromatography/MS/MS method for glycopeptide antibiotics determination in human plasma
【24h】

Analytical Quality by Design-based development and validation of ultra pressure liquid chromatography/MS/MS method for glycopeptide antibiotics determination in human plasma

机译：基于设计的开发和验证超压液相色谱/ MS / MS方法的分析质量，用于人血浆中的糖肽抗生素测定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aim: An ultra pressure liquid chromatography (UPLC)/MS/MS method for vancomycin and teicoplanin determination in human plasma was developed in accordance with analytical quality by design (AQbD) concept and fully validated. Materials & methods: Chromatographic separation was performed on ACQUITY UPLC C-18 charge surface hybrid (CSH) column (2.1 mm x 50 mm, 1.7 mu m particle size) in gradient mode and the mobile phase consisted of 0.1% formic acid in water and pure acetonitrile. The experimental design methodology was used for the definition of optimal chromatographic and protein precipitation conditions. Results: The linearity ranges were 0.05-10 mu g ml(-1) for vancomycin and 0.5-200 mu g ml(-1) for total teicoplanin. The relative standard deviations for precision estimation were below 15% and the accuracy was within 85-115% for all quality control levels. Conclusion: The method was utilized for glycopeptide antibiotics bioanalysis.
机译：目的：按照设计（AQBD）概念和完全验证，通过设计（AQBD）概念的分析质量开发了用于万古霉素和人血浆中的超压液相色谱（UPLC）/ MS / MS / MS方法。 材料和方法：在梯度模式下对诸如UPLC C-18电荷表面杂合（CSH）柱（CSH）柱（CSH）柱（2.1mm×50mm，1.7μm粒度）进行色谱分离，流动相在水中组成0.1％甲酸 纯乙腈。 实验设计方法用于定义最佳色谱和蛋白质沉淀条件。 结果：对于万古霉素，线性范围为0.05-10μgmml（-1），用于总Teicoplanin的0.5-200 mm g ml（-1）。 精度估计的相对标准偏差低于15％，对所有质量控制水平的精度不到85-115％。 结论：该方法用于糖肽抗生素生物分析。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号