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Large-molecule quantification: sensitivity and selectivity head-to-head comparison of triple quadrupole with Q-TOF

机译：大分子量化：具有Q-TOF的三重四极针的敏感性和选择性头部比较

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Background: Bioanalysts are continuously looking for innovative ideas or instruments to increase the sensitivity and selectivity of their assays. Research for better mass spectrometers is becoming crucial with the emerging trend of large-molecule quantification. This study lists the different advantages of high-resolution MS (HRMS) over standard triple quadrupole instruments and proposes basic guidelines on how to use HRMS for large-molecule quantification in a regulated environment. Results: A direct comparison between HRMS and triple quadrupole instruments for the quantification of six different model peptides (desmopressin, calcitonin, enfuvirtide, exenatide, glucagon and somatostatin) was completed. The HRMS instrument, when used specifically for targeted quantification ('quant/quant'), showed equivalent or better sensitivity for all compounds tested. Conclusion: This paper demonstrates that the use of a HRMS instrument in a regulated environment is a viable technique for quantification of large molecules. The latter was able to allow flexibility and selectivity to adapt the specificity of each assay with sensitivity comparable to the triple quadrupole instrument.
机译：背景：生物分析人不断寻找创新的思想或仪器，以提高其测定的灵敏度和选择性。对更好的质谱仪的研究与大分子量化的新出现趋势变得至关重要。本研究列出了高分辨率MS（HRMS）对标准三重四极杆仪器的不同优点，并提出了关于如何在受监管环境中使用HRMS进行大分子量化的基本指南。结果：HRMS与三重四极杆仪器之间的直接比较，用于定量六种不同的模型肽（去氨加压素，降钙素，苯二甲酸盐，八肽，胰高血糖素和生长抑制素）。用于专门用于靶向定量的HRMS仪器（'量子/量子'），显示出所有测试的所有化合物的等效或更好的敏感性。结论：本文表明，在调节环境中使用HRMS仪器是用于量化大分子的可行技术。后者能够允许灵活性和选择性，以适应每个测定的特异性，其具有与三重四极仪器相当的灵敏度。

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