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首页> 外文期刊>Bioanalysis >An oligonucleotide bioanalytical LC-SRM methodology entirely liberated from ion-pairing
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An oligonucleotide bioanalytical LC-SRM methodology entirely liberated from ion-pairing

机译：一种寡核苷酸生物分析LC-SRM方法，完全从离子配对中释放

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Aim: Reliable quantitative LC-MS methodology has been established and validated for an oligonucleotide in plasma in a fresh and unique fashion, free of ion-pairing reagents and the various associated deleterious effects from primary solution preparation through sample preparation and extraction to the LC-MS analytical end point, offering a highly selective mixed-mode solid-phase extractionwith hydrophilic-interaction liquid chromatography as the chromatographic element prior to SRM detection. Results: Inter- and intra-assay accuracy and precision ranged from 97.9 to 111% and 2.75 to 9.66%, respectively. Recoveries of 50% were attained, and there was no significant matrix effect manifestation. Conclusion: The method demonstrated rugged performance and reliability under the optimized conditions, indicating a possible exciting new avenue, free of ion-pairing, for general application in oligonucleotide quantitative LC-MS.
机译：目的：以新鲜和独特的方式，以新鲜和独特的方式为血浆中的寡核苷酸的寡核苷酸，从原发性溶液制备通过样品制备和提取到LC- MS分析终点，提供高度选择性的混合模式固相提取，使亲水性相互作用液相色谱作为在SRM检测之前作为色谱元素。 结果：测定的间间和分析精度和精度分别为97.9至111％和2.75至9.66％。 达到50％的回收率，没有明显的基质效应表现。 结论：该方法在优化条件下表现出粗糙的性能和可靠性，表明可能令人兴奋的新大道，没有离子配对，用于寡核苷酸定量LC-MS中的一般应用。

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