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首页> 外文期刊>Cytometry, Part A: the journal of the International Society for Analytical Cytology >Texture-Based Segmentation and a New Cell Shape Index for Quantitative Analysis of Cell Spreading in AFM Images
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Texture-Based Segmentation and a New Cell Shape Index for Quantitative Analysis of Cell Spreading in AFM Images

机译：基于纹理的分割和新的细胞形状指数，用于定量分析AFM图像中的细胞扩散

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摘要

A new cell shape index is defined for use with atomic force microscopy height images of cell cultures. The new cell shape index reveals quantitative cell spreading information not included in a conventional cell shape index. A supervised learning-based cell segmentation algorithm was implemented by texture feature extraction and a multi-layer neural network classifier. The texture feature sets for four different culture surfaces were determined from the gray level co-occurrence matrix and local statistics texture models using two feature selection algorithms and by considering computational cost. The quantitative morphometry of quiescent-like and reactive-like cerebral cortical astrocytes cultured on four different culture environments was investigated using the new and conventional cell shape index. Inclusion of cell spreading with stellation information through use of the new cell shape index was shown to change biomedical conclusions derived from conventional cell shape analysis based on stellation alone. The new CSI results showed that the quantitative astrocyte spreading and stellation behavior was induced by both the underlying substrate and the immunoreactivity of the astrocytes. (C) 2015 International Society for Advancement of Cytometry
机译：定义了一种新的细胞形状指数，用于细胞培养的原子力显微镜高度图像。新的细胞形状指数揭示了常规细胞形状指数中未包括的定量细胞扩散信息。通过纹理特征提取和多层神经网络分类器，实现了基于监督学习的细胞分割算法。使用两个特征选择算法并考虑计算成本，从灰度共生矩阵和局部统计纹理模型确定了四个不同培养表面的纹理特征集。使用新的和常规的细胞形状指数，研究了在四种不同培养环境下培养的静态和反应性大脑皮质星形胶质细胞的定量形态。通过使用新的细胞形状指数将细胞扩散与星状信息包括在一起，已显示出改变了仅基于星状的常规细胞形状分析得出的生物医学结论。新的CSI结果表明，星形胶质细胞的定量扩散和星状行为是由潜在的底物和星形胶质细胞的免疫反应性诱导的。 （C）2015国际细胞计数学会

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