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首页> 外文期刊>Cytologia : International journal of cytology >Sex-Specific Cell Fusion Pattern of Isogametes in Marine Green Alga, Acetabularia caliculus (Ulvophyceae, Chlorophyta)
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Sex-Specific Cell Fusion Pattern of Isogametes in Marine Green Alga, Acetabularia caliculus (Ulvophyceae, Chlorophyta)

机译：海洋绿藻，轮生棘角（Ulvophyceae，Chlorophyta）中同配子的性别特异性细胞融合模式

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Using field emission scanning electron microscopy (FE-SEM) and fluorescence microscopy, the respective relations between the arrangements of the gamete cell fusion site and the inheritance pattern of chloroplast DNA (cp-DNA) as a sex-specific marker were studied for the isogamous dasycladalean alga Acetabularia caliculus. The gamete had two flagella elongated from the cell anterior. One oval-shaped eyespot situated on the cell posterior was visualized using FE-SEM. When the gametes belonging to the opposite mating types were mixed together, the two gametes aligned side-by-side at their lateral side and fused to form a quadriflagellate planozygote. In the planozygote, the two flagella from the opposite mating type gametes lay side-by-side and two eyespots aligned on the same side of the cell, suggesting opposite positioning of the cell fusion sites between two parental gametes. To confirm whether the gamete fusion pattern observed in the planozygotes was a result of sex-specific fusion or not, the inheritance pattern of cp-DNA was observed using fluorescence microscopy after staining with DAPI concomitantly with the cell fusion pattern. When the eyespots were used as positional marker and were viewed from the surface that included the eyespots, the chloroplast of one parental gamete was found to contain cp-DNAs, although cp-DNAs were not detected in the chloroplast derived from another parental gamete. These results suggest that the gamete fusion pattern observed by FE-SEM was a result of sex-specific fusion.
机译：利用场发射扫描电子显微镜（FE-SEM）和荧光显微镜，研究了配子细胞融合位点的排列与作为性别特异性标记的叶绿体DNA（cp-DNA）的遗传模式之间的关系。 dasycladalean藻Acetabularia caliculus。配子有两个鞭毛从细胞前部拉长。使用FE-SEM观察位于细胞后部的一个椭圆形眼点。当属于相反交配类型的配子混合在一起时，两个配子在其侧面并排排列并融合在一起，形成了四鞭毛的扁平合子。在扁平虫中，来自相反交配型配子的两个鞭毛并排放置，并且两个眼点对准细胞的同一侧，表明两个亲代配子之间的细胞融合位点相反。为了确认在扁平卵子中观察到的配子融合模式是否是性别特异性融合的结果，在用DAPI染色并与细胞融合模式同时染色后，使用荧光显微镜观察了cp-DNA的遗传模式。当使用眼点作为位置标记并从包括眼点的表面观察时，发现一个亲代配子的叶绿体含有cp-DNA，尽管在衍生自另一亲子配子的叶绿体中未检测到cp-DNA。这些结果表明，通过FE-SEM观察到的配子融合模式是性别特异性融合的结果。

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