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首页> 外文期刊>Bulletin of the European Association of Fish Pathologists >First description of health status and associated bacterial diversity from non-native Chinook salmon (Oncorhynchus tshawytscha, Walbaum 1792) naturalised in a large river system from southern Chile
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First description of health status and associated bacterial diversity from non-native Chinook salmon (Oncorhynchus tshawytscha, Walbaum 1792) naturalised in a large river system from southern Chile

机译：从智利南部的大型河流系统中归化的健康状况和相关细菌多样性的首先描述

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Chinook salmon (Oncorhynchus tshawytscha Walbaum, 1792) was naturalised in many river systems from South America following aggressive introduction efforts. This study investigated the health status and bacterial diversity of Chinook salmon from the Tolten River in southern Chile. Ribosomal and gene-coding markers were sequenced to establish the presence/absence of pathogens common to farmed salmonids. Pathological examination of 30 adult Chinook salmon showed no internal/ external clinical signs of disease. Nine specimens were used for microbiological assessments, with all bacterial isolates identified to the genus or species level using 16S rDNA sequencing. Tissue samples from the remaining 21 specimens were subjected to PCR analyses for known pathogens. Sequencing of 16S rRNA resulted in 48.48% (32 of 66) of the obtained isolates matching to the Vibrio, Psychrobacter, and Pseudomonas genera. Exiguabacterium, Arthrobacter, Aliivibrio, Shewanella, and Sphingobacterium genera were represented to a lesser extent (3-5 isolates each). Of the 66 isolates, 27, 22, and 17 were respectively obtained from kidney, spleen, and liver samples. Positive molecular amplifications were obtained for 11 of the 63 tissue samples (17.4%), with 9 being Vibrio ordalii, 1 being Piscirickettsia salmonis, and 1 being Aeromonas salmonicida. However, when RT-PCR protocols for viral diagnostics were applied (i.e. IPNV and ISAV); no amplification products were detected. Despite a wide range of detected bacteria, continued research is needed to determine if Chinook salmon act as disease vectors or if bacterial colonisation is normal and asymptomatic. The presence of common aquaculture-related pathogens suggests the assessed Chinook salmon population may have recently originated from captive broodstock, which would be consistent with anecdotal information and genetic data.
机译：Chinook Salmon（Oncorhynchus Tshawytscha Walbaum，1792）在南美洲的许多河流系统中被归化，后者在积极的介绍努力之后。本研究调查了智利南部托尔顿河的奇努克鲑鱼的健康状况和细菌多样性。测序核糖体和基因编码标志物，以确定养殖鲑鱼类共同的病原体的存在/不存在。 30名成人奇努克鲑鱼的病理学检查显示没有内部/外部疾病的临床迹象。九个样本用于微生物评估，所有细菌分离株使用16S rDNA测序鉴定为属或物种水平。剩余21种样本的组织样品对已知病原体进行PCR分析。 16S rRNA的测序导致所得分离物与vibrio，心理杆菌和假单胞菌属匹配的48.48％（32个）。引用，关节杆菌，嗜酸剂，肺杆菌和鞘脉属于较小程度（每次3-5分离株）。在66个分离物中，分别从肾，脾和肝脏样品获得27,22和17个。得到正分子扩增的63个组织样品（17.4％），9是Quirio ordalii，1是Piscirickettsia salmonis，1是Aeromonas Salmonicida。但是，当应用用于病毒诊断的RT-PCR方案（即IPNV和ISAV）;未检测到扩增产物。尽管有广泛的检测到细菌，但需要持续的研究来确定Chinook鲑鱼是否充当疾病载体，或者如果细菌殖民化是正常的并且无症状。常见水产养殖相关病原体的存在表明，评估的Chinook鲑鱼群最近可能源于俘虏亲属，这将与轶事信息和遗传数据一致。

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