• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Calcified tissue international. >Potential of Osteoblastic Cells Derived from Bone Marrow and Adipose Tissue Associated with a Polymer/Ceramic Composite to Repair Bone Tissue
【24h】

Potential of Osteoblastic Cells Derived from Bone Marrow and Adipose Tissue Associated with a Polymer/Ceramic Composite to Repair Bone Tissue

机译：与聚合物/陶瓷复合材料相关的骨髓和脂肪组织衍生自骨髓和脂肪组织的潜力以修复骨组织

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

One of the tissue engineering strategies to promote bone regeneration is the association of cells and biomaterials. In this context, the aim of this study was to evaluate if cell source, either from bone marrow or adipose tissue, affects bone repair induced by osteoblastic cells associated with a membrane of poly(vinylidene-trifluoroethylene)/barium titanate (PVDF-TrFE/BT). Mesenchymal stem cells (MSC) were isolated from rat bone marrow and adipose tissue and characterized by detection of several surface markers. Also, both cell populations were cultured under osteogenic conditions and it was observed that MSC from bone marrow were more osteogenic than MSC from adipose tissue. The bone repair was evaluated in rat calvarial defects implanted with PVDF-TrFE/BT membrane and locally injected with (1) osteoblastic cells differentiated from MSC from bone marrow, (2) osteoblastic cells differentiated from MSC from adipose tissue or (3) phosphate-buffered saline. Luciferase-expressing osteoblastic cells derived from bone marrow and adipose tissue were detected in bone defects after cell injection during 25 days without difference in luciferin signal between cells from both sources. Corroborating the in vitro findings, osteoblastic cells from bone marrow combined with the PVDF-TrFE/BT membrane increased the bone formation, whereas osteoblastic cells from adipose tissue did not enhance the bone repair induced by the membrane itself. Based on these findings, it is possible to conclude that, by combining a membrane with cells in this rat model, cell source matters and that bone marrow could be a more suitable source of cells for therapies to engineer bone.
机译：促进骨再生的组织工程策略之一是细胞和生物材料的关联。在这种情况下，本研究的目的是评估来自骨髓或脂肪组织的细胞来源，影响由与聚（亚乙烯基 - 三氟乙烯）/钛酸钡的膜相关的骨细胞细胞诱导的骨修复（PVDF-TRFE / BT）。从大鼠骨髓和脂肪组织中分离间充质干细胞（MSC），并通过检测几种表面标记来表征。而且，在成骨状况下培养了两种细胞群，观察到来自骨髓的MSC比来自脂肪组织的MSC更容易成骨。在植入PVDF-TRFE / BT膜的大鼠颅骨缺陷中评价骨修复，并局部注射由MSC的来自MSC的骨髓细胞，（2）从脂肪组织或（3）磷酸盐分化的成骨细胞缓冲盐水。在25天期间，在细胞注射后，在骨缺陷中检测到源自骨髓和脂肪组织的荧光素酶 - 表达骨细胞细胞，从而无差异来自两个来源的细胞之间的荧光素信号。证实体外发现，来自骨髓的成骨细胞与PVDF-TRFE / BT膜联合增加骨形成，而来自脂肪组织的骨细胞细胞并未增强由膜本身诱导的骨骼修复。基于这些发现，可以得出结论，通过将膜与该大鼠模型中的细胞组合，细胞源重要，并且骨髓可以是更合适的细胞来源，用于工程师骨骼。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号