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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Beta-sitosterol upregulated paraoxonase-1 via peroxisome proliferator-activated receptor-gamma in irradiated rats
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Beta-sitosterol upregulated paraoxonase-1 via peroxisome proliferator-activated receptor-gamma in irradiated rats

机译：β-谷甾醇通过过氧化物体增殖物激活的受体-γ在辐照大鼠中上调定律酶-1

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摘要

This study was designed to evaluate the effect of beta-sitosterol (BS) on the peroxisome proliferator-activated receptor gamma (PPAR-gamma) gene expression role in the activity of paraoxonase (PON-1) enzyme in oxidative stress status of irradiated rats. Animals were exposed to whole body gamma-radiation single dose 6 Gy and received BS dose (40 mg.(kg body mass)(-1).day(-1), orally). In liver tissue, gene expression of PPAR-gamma ligand was determined. Oxidative stress marker (malondialdehyde, MDA) and antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), PON-1, and arylesterase (ARE)) were assayed in serum and liver tissue. Also, serum lipid profile (cholesterol, triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), and high-density lipoprotein cholesterol (HDL-c)) was measured. In irradiated animals that received BS, expression of PPAR-gamma ligand increase significantly associated with increase in PON-1 and ARE enzyme activities. Also, the activities of SOD, CAT enzymes, and HDL-c levels display elevation. By contrast, significant decrease in MDA content, cholesterol, TG, and LDL-c levels were revealed after BS administration. Our findings in this study provide the evidence that BS has radio-protective effect via regulating the gene expression of PPAR-gamma, causing an increase in PON-1 and ARE enzyme activities. This action of BS is due to its free radical scavenging properties, antioxidant effect, lowering of cholesterol, and PPAR-gamma agonist properties.
机译：该研究旨在评估β-谷甾醇（BS）对辐射大鼠氧化应激状态致氧酶（PON-1）酶活性的过氧化物体增殖物激活受体γ（PPAR-GAMMA）基因表达的作用。将动物暴露于全身γ-辐射单剂量6 Gy并接受BS剂量（40mg（kg体重）（ - 1）.ney，口服）。在肝组织中，测定PPAR-Gamma配体的基因表达。在血清和肝组织中测定氧化应激标记物（丙二醛，MDA）和抗氧化酶活性（超氧化物歧化酶（SOD），过氧化氢酶（猫），PON-1和芳基酯酶（是）。此外，测量血清脂质曲线（胆固醇，甘油三酯（Tg），低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C））。在接受BS的辐射动物中，PPAR-γ配体的表达随着PON-1的增加而显着相关，并且是酶活性。此外，SOD，猫酶和HDL-C水平的活性显示升高。相比之下，在BS给药后发现了MDA含量，胆固醇，TG和LDL-C水平的显着降低。我们在本研究中的研究结果提供了通过调节PPAR-γ的基因表达，BS具有无线电保护作用的证据，导致PON-1增加并且是酶活性。 BS的这种作用是由于其自由基清除性质，抗氧化效果，降低胆固醇和PPAR-γ激动剂性能。

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