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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Adaptation of mitochondrial expression and ATP production in dedifferentiating vascular smooth muscle cells
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Adaptation of mitochondrial expression and ATP production in dedifferentiating vascular smooth muscle cells

机译：改性线粒体的表达和ATP生产在去除湿血管平滑肌细胞中

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摘要

Atherosclerosis is one of the leading causes of morbidity and mortality in the Western world. Although the clinical manifestations of this disease are well documented, the etiology and progression remain to be fully understood. Recently, the mitochondria have been implicated in important cellular processes involved in development of atherosclerosis. Despite the link between mitochondria and atherosclerosis, early-phase mechanisms of the disease have yet to be elucidated. The aim of this project was to explore the role of mitochondria in vascular smooth muscle (VSMC) dedifferentiation. A murine in vitro model, involving organ culture of aortic tissue in serum-free media, was used. Mitochondrial function was measured by high-resolution respirometry. Proteins associated with the VSMC phenotype switch, as well as mitochondrial density, were assessed by immunoblotting. The findings show that intrinsic mitochondrial Complex I activity is significantly upregulated during VSMC dedifferentiation. Diminished coupling between phosphorylation and oxidation was also found, indicating a greater ADP:ATP ratio. This data suggests increased leak in the electron transport chain and altered mitochondrial function specifically at Complex I. This project provides important information regarding the role of mitochondria in the early atherosclerotic process and that detectable changes in mitochondrial function and expression are related to VSMC dedifferentiation.
机译：动脉粥样硬化是西方世界中发病率和死亡率的主要原因之一。虽然这种疾病的临床表现良好，但仍有待全面理解的病因和进展。最近，线粒体涉及涉及动脉粥样硬化的发展的重要细胞过程。尽管线粒体和动脉粥样硬化之间的联系，但疾病的早期机制尚未阐明。该项目的目的是探讨线粒体的作用在血管平滑肌（VSMC）去消除期。使用涉及无血清培养基中的主动脉组织器官培养的鼠体外模型。通过高分辨率呼​​吸测定测量线粒体功能。通过免疫印迹评估与VSMC表型开关相关的蛋白质以及线粒体密度。研究结果表明，在VSMC Deffifferiation期间，内在的线粒体复合物I活性显着上调。还发现磷酸化与氧化之间的耦合减少，表明ADP：ATP比例更大。该数据表明，在复合体I.特异性的电子传输链中泄漏和改变的线粒体功能。该项目提供了关于线粒体在早期动脉粥样硬化过程中的作用的重要信息，并且线粒体功能和表达的可检测变化与VSMC Deffifetiation有关。

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