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首页> 外文期刊>Cytometry: The Journal of the Society for Analytical Cytology >Growth kinetics of algal populations exsymbiotic from Paramecium bursaria by flow cytometry measurements
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Growth kinetics of algal populations exsymbiotic from Paramecium bursaria by flow cytometry measurements

机译：流式细胞仪测量法测定草履虫中共生藻类种群的生长动力学

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Background: The ciliate Paramecium bursaria normally exists as a green paramecium system because each animal cell carries several hundred, unicellular, green, algal cells in its cytoplasm. One of the remarkable and poorly understood pecularities of this system is the steady state in the number of algae per protozoan cell. A major point in the study of mechanisms governing the persistence of symbiont numbers is adequate understanding of the algal life cycle. Methods: Asynchronously growing cell populations of several algal strains (SA-1, SA-3, and SA-3) exsymbiotic from P. bursaria were characterized by flow cytometry. Algal endogenous chlorophyll and DNA contents were monitored to analyze cell growth kinetics at logarithmic and stationary culture phases. Cell. sorting visualized the morphology of algae corresponding to the hyperhaploid (2C and 4C) DNA peaks. Results: Cell-division cycle-dependent changes in chlorophyll and DNA content distributions were most dramatic in logarithmically growing algal populations tan increase in the number of S-phase cells and cells with more chlorophyll), which are thought to be associated with accelerated DNA and chlorophyll metabolism in log-phase algal cultures. Upon reaching the stationary phase of growth, algal populations distinctly showed, in addition to one haploid (1C) DNA peak, two hyperhaploid peaks (2C and 4C) corresponding mainly to cells with two and four nuclei, respectively. Conclusions: Growth characteristics of algae exsymbiotic from P. bursaria monitored by flow cytometry provide valuable information for the analysis of the algal life cycle, which is important for understanding the regulation mechanisms of symbiont numbers. (C) 2001 Wiley-Liss, Inc. [References: 22]
机译：背景：纤毛化生草履虫通常以绿色草履虫系统存在，因为每个动物细胞在其细胞质中都携带数百个单细胞绿色藻类细胞。该系统的显着和鲜为人知的特征之一是每个原生动物细胞藻类数量的稳定状态。研究控制共生体数目持久性的机制的一个重点是对藻类生命周期的充分理解。方法：通过流式细胞术对法氏囊法共生的几种共生藻类菌株（SA-1，SA-3和SA-3）的异步生长细胞群进行了表征。监测藻类内源性叶绿素和DNA含量，以分析对数和静止培养阶段的细胞生长动力学。细胞。排序显示了与超单倍体（2C和4C）DNA峰相对应的藻类形态。结果：细胞分裂周期依赖性的叶绿素和DNA含量分布变化在对数生长的藻类种群中最为显着，S期细胞和叶绿素含量更高的细胞数量增加），这被认为与DNA加速和对数期藻类培养物中的叶绿素代谢。到达生长的稳定期后，除一个单倍体（1C）DNA峰外，藻类种群还清楚地显示了两个超单倍体峰（2C和4C），分别对应于具有两个和四个核的细胞。结论：流式细胞仪检测法氏囊藻共生藻的生长特征，为藻类生命周期的分析提供了有价值的信息，对于了解共生体数量调控机制具有重要意义。 （C）2001 Wiley-Liss，Inc. [参考：22]

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