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首页> 外文期刊>Cytometry: The Journal of the Society for Analytical Cytology >Magnetic field design for selecting and aligning immunomagnetic labeled cells
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Magnetic field design for selecting and aligning immunomagnetic labeled cells

机译：用于选择和排列免疫磁性标记细胞的磁场设计

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Background: Recently we introduced the CellTracks cell analysis system, in which samples are prepared based on a combination of immunomagnetic selection, separation, and alignment of cells along ferromagnetic lines. Here we describe the underlying magnetic principles and considerations made in the magnetic field design to achieve the best possible cell selection and alignment of magnetically labeled cells. Materials and Methods: Computer simulations, in combination with experimental data, were used to optimize the design of the magnets and Ni lines to obtain the optimal magnetic configuration. Results: A homogeneous cell distribution on the upper surface of the sample chamber was obtained with a magnet where the pole faces were tilted towards each other. The spatial distribution of magnetically aligned objects in between the Ni lines was dependent on the ratio of the diameter of the aligned object and the line spacing, which was tested with magnetically and fluorescently labeled 6 mum polystyrene beads. The best result was obtained when the line spacing was equal to or smaller than the diameter of the aligned object. Conclusions: The magnetic gradient of the designed permanent magnet extracts magnetically labeled cells from any cell suspension to a desired plane, providing a homogeneous cell distribution. In addition, it magnetizes ferromagnetic Ni lines in this plane whose additional local gradient adds to the gradient of the permanent magnet. The resultant gradient aligns the magnetically labeled cells first brought to this plane, This combination makes it possible, in a single step, to extract and align cells on a surface from any cell suspension. (C) 2002 Wiley-Liss, Inc. [References: 15]
机译：背景：最近，我们引入了CellTracks细胞分析系统，该系统基于免疫磁性选择，分离和沿铁磁性线排列的细胞组合来制备样品。在这里，我们描述了基本的磁性原理和在磁场设计中进行的考虑，以实现最佳的细胞选择和磁性标记细胞的排列。材料和方法：计算机模拟结合实验数据，用于优化磁体和Ni线的设计，以获得最佳的磁配置。结果：用磁铁将样品室的上表面均匀分布，其中的磁极表面彼此相对倾斜。 Ni线之间的磁性对齐对象的空间分布取决于对齐对象的直径与行间距的比率，这是用磁性和荧光标记的6毫米聚苯乙烯珠测试的。当行距等于或小于对齐对象的直径时，可获得最佳结果。结论：设计的永磁体的磁梯度将磁性标记的细胞从任何细胞悬液中提取到所需平面，从而提供均匀的细胞分布。此外，它会在此平面中磁化铁磁Ni线，其附加局部梯度会增加永磁体的梯度。最终的梯度使首先进入该平面的磁性标记细胞对齐。这种结合使得在单个步骤中可以从任何细胞悬液中提取并对齐表面上的细胞。 （C）2002 Wiley-Liss，Inc. [参考：15]

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