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首页> 外文期刊>Cytometry: The Journal of the Society for Analytical Cytology >Evaluation of a universal template for single-platform absolute T-lymphocyte subset enumeration
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Evaluation of a universal template for single-platform absolute T-lymphocyte subset enumeration

机译:单平台绝对T淋巴细胞亚群计数通用模板的评估

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The single-platform absolute T-lymphocyte subset analysis was evaluated utilizing a universal protocol in a Canadian multicenter study with the collaboration of the members of the Canadian HIV Trials Network (CTN). Participants used flow cytometers and reagents of their choice for labeling and lysing whole blood. Over a 2-year period, CTN laboratories performed single-platform absolute T-lymphocyte subset enumerations on fresh and commercial stabilized blood products using commercially available microfluorospheres TruCount and Flow-Count. This multicenter evaluation demonstrated that the application of a universal template for single-platform analysis provides a generic approach that embraces a wide array of immunophenotyping settings available in clinical laboratory. Cytometry (Clin. Cytometry) 50:62-68, 2002. (C) 2002 Wiley-Liss, Inc. [References: 15]
机译:在加拿大多中心研究中,与加拿大HIV试验网络(CTN)成员合作,使用通用方案评估了单平台绝对T淋巴细胞亚群分析。参与者使用流式细胞仪和他们选择的试剂来标记和裂解全血。在2年的时间里,CTN实验室使用市售的微球体TruCount和Flow-Count对新鲜和商业稳定的血液产品进行了单平台绝对T淋巴细胞亚群计数。这项多中心评估表明,通用模板在单平台分析中的应用提供了一种通用方法,该方法涵盖了临床实验室中可用的多种免疫表型设置。细胞计数法(Clin。Cytometry)50:62-68,2002.(C)2002 Wiley-Liss,Inc. [参考:15]

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