• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cytoskeleton >Mapping cytoskeletal protein function in cells by means of nanobodies
【24h】

Mapping cytoskeletal protein function in cells by means of nanobodies

机译：通过纳米抗体定位细胞中的细胞骨架蛋白功能

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nanobodies or VHHs are single domain antigen binding fragments derived from heavy-chain antibodies naturally occurring in species of the Camelidae. Due to their ease of cloning, high solubility and intrinsic stability, they can be produced at low cost. Their small size, combined with high affinity and antigen specificity, enables recognition of a broad range of structural (undruggable) proteins and enzymes alike. Focusing on two actin binding proteins, gelsolin and CapG, we summarize a general protocol for the generation, cloning and production of nanobodies. Furthermore, we describe multiple ways to characterize antigen-nanobody binding in more detail and we shed light on some applications with recombinant nanobodies. The use of nanobodies as intrabodies is clarified through several case studies revealing new cytoskeletal protein properties and testifying to the utility of nanobodies as intracellular bona fide protein inhibitors. Moreover, as nanobodies can traverse the plasma membrane of eukaryotic cells by means of the enteropathogenic E. coli type III protein secretion system, we show that in this promising way of nanobody delivery, actin pedestal formation can be affected following nanobody injection.
机译：纳米抗体或VHH是单域抗原结合片段，其衍生自天然存在于骆驼科动物物种中的重链抗体。由于它们易于克隆，高溶解度和固有稳定性，因此可以低成本生产。它们的体积小，结合了高亲和力和抗原特异性，可以识别各种结构性（非药物性）蛋白质和酶。着眼于两种肌动蛋白结合蛋白，凝溶胶蛋白和CapG，我们总结了生成，克隆和生产纳米抗体的通用协议。此外，我们描述了表征抗原-纳米抗体结合的多种方法，并且更详细地介绍了重组纳米抗体的一些应用。通过一些案例研究阐明了纳米抗体作为体内抗体的用途，这些案例揭示了新的细胞骨架蛋白特性，并证明了纳米抗体作为细胞内善意蛋白抑制剂的效用。此外，由于纳米抗体可以通过肠致病性大肠杆菌III型蛋白质分泌系统穿越真核细胞的质膜，因此我们证明，以这种有前途的纳米抗体递送方式，注射纳米抗体后肌动蛋白基体的形成会受到影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号