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首页> 外文期刊>Balkan journal of medical genetics: BJMG >INVESTIGATION OF CIRCULATING SERUM microRNA-328-3p AND microRNA-3135a EXPRESSION AS PROMISING NOVEL BIOMARKERS FOR AUTISM SPECTRUM DISORDER
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INVESTIGATION OF CIRCULATING SERUM microRNA-328-3p AND microRNA-3135a EXPRESSION AS PROMISING NOVEL BIOMARKERS FOR AUTISM SPECTRUM DISORDER

机译：循环血清MicroRNA-328-3P和MicroRNA-3135A表达作为自闭症谱系疾病的有前途的新型生物标志物

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Circulating microRNAs (miRNAs) are emerging as promising diagnostic biomarkers for autism spectrum disorder (ASD), but their usefulness for detecting ASD remains unclear. Nowadays, development of promising biomarkers for ASD remains a challenge. Recently, dysregulation of the miRNAs expression in postmortem brain tissue, serum and peripheral blood, have been associated with ASD. Circulating miRNAs are known to be secreted by a number of different cells and can interpose delivery of information into receiver cells, thus affecting their functions. Based on this fact, it is supposed that serum miRNAs could be a novel class of biomarkers for prognosis or diagnosis of pathological disorders including ASD. In the current research, we investigated whether the expression patterns of circulating miRNAs showed dysregulation in subjects diagnosed with ASD. Expression levels of serum miR-328-3p and miR-3135a were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) method of subjects diagnosed with ASD in comparison with healthy control subjects. Our data showed that miR-328-3p and miR-3135a were substantially down-regulated in ASD patients than in those of healthy control subjects. Moreover, target gene analysis of altered serum miRNAs displayed that these molecules targeted 162 genes denoted as unique validated targets in the miRWalk database, 71 of which appear to participate in biological pathways involved in synaptic pathways and neurodegenerative condition such as Alzheimer, Huntington and Parkinson diseases. Finally, the results strongly suggested that dys-regulated serum miRNAs might be involved in molecular pathways associated with ASD and miR-328-3p and miR-3135a have the potential to be promising novel biomarkers for ASD.
机译：循环的MicroRNA（miRNA）是作为自闭症谱系疾病（ASD）的有前途的诊断生物标志物，但它们对检测ASD的有用性仍不清楚。如今，为ASD开发有前途的生物标志物仍然是一个挑战。最近，在后期脑组织，血清和外周血中的miRNA表达的失调已经与ASD相关。已知循环miRNA被许多不同的电池分泌，并且可以将信息传送到接收器单元中，从而影响其功能。基于这一事实，假设血清MiRNA可以是一种新型一类生物标志物，用于预后或诊断包括ASD的病理疾病。在目前的研究中，我们研究了循环miRNA的表达模式是否在被诊断患有ASD的受试者中显示出缺血剂。通过定量逆转录聚合酶链反应（QRT-PCR）对与健康对策相比，通过定量逆转录聚合酶链反应（QRT-PCR）方法分析了血清miR-328-3p和miR-3135a的表达水平。我们的数据显示，MIR-328-3P和MIR-3135A在ASD患者中基本上下调而不是健康对照受试者的数据。此外，改变血清miRNA的靶基因分析显示，这些分子靶向162个基因表示为MiRWalk数据库中的独特验证靶标，其中71个似乎参与突触途径和神经变性病症（如Alzheimer，Huntington和Parkinson疾病）的生物途径。最后，结果强烈建议，功能调节的血清miRNA可以参与与ASD相关的分子途径，MIR-328-3P和MIR-3135A具有对ASD的新的生物标志物有可能成为有望的新型生物标志物。

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