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首页> 外文期刊>Cancer prevention research. >Breast Hormone Concentrations in Random Fine-Needle Aspirates of Healthy Women Associate with Cytological Atypia and Gene Methylation
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Breast Hormone Concentrations in Random Fine-Needle Aspirates of Healthy Women Associate with Cytological Atypia and Gene Methylation

机译：乳腺激素浓度在随机细针起伏的健康妇女与细胞学类别和基因甲基化术语联系起来

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Sex steroid hormones contribute to breast cancer development, but data on concentrations of these within breast tissue are limited. We performed simultaneous multiparameter measurement of breast sex steroids, breast epithelial cytology, and DNA methylation in 119 healthy women (54 pre- and 65 postmenopausal) without a history of breast cancer. Random fine-needle aspiration (rFNA) of the breast was performed simultaneously with blood collection. Breast samples were analyzed by LC/MS-MS for estrone, estradiol, progesterone, androstenedione, and testosterone. Blood samples were assayed for estradiol and progesterone by immunoassay. Cytomorphology was classified using the Masood Score, and DNA methylation of eight genes was analyzed using quantitative multiplexed methylation-specific PCR, and expressed as the cumulative methylation index (CMI). Serum and breast concentrations of estradiol and progesterone showed significant correlation (Spearman r = 0.34, P-adj = 0.001 and - 0.69, P-adj 0.0006, respectively). Progesterone concentration was significantly higher in the premenopausal breast (P-adj/ 0.0008), and showed a luteal surge. Breast estrone and estradiol concentrations did not differ significantly by menopause, but androstenedione concentration was higher in the breasts of postmenopausal women (P = 0.026 and P-adj = 0.208). Breast androgens were significantly correlated with breast density (Spearman r = 0.27, P-adj = 0.02 for testosterone) and CMI (Spearman r = 0.3, P-adj = 0.038 for androstenedione). Our data indicate that future larger studies of breast steroid hormones along with other parameters are feasible. Significant associations of breast androgen concentrations with breast density and gene methylation warrant future study. (C) 2018 AACR.
机译：性类固醇激素有助于乳腺癌发育，但是乳腺组织内这些浓度的数据有限。我们在119名健康女性（54例和65例后期）中的乳腺性类固醇，乳腺上皮细胞学和DNA甲基化的同时进行多次多道琼布测量，没有乳腺癌的历史。乳腺的随机细针抽吸（RFNA）与血液收集同时进行。通过LC / MS-MS进行乳腺样品，用于Estrone，雌二醇，孕酮，雄甾酮和睾酮。通过免疫测定法为雌二醇和孕酮测定血液样品。使用Masop评分进行分类细胞形态，使用定量复用甲基化特异性PCR分析8个基因的DNA甲基化，并表示为累积甲基化指数（CMI）。雌二醇和孕酮的血清和乳腺浓度显示出显着的相关性（Spearman r = 0.34，p-adj = 0.001和-0.69，p-adj <0.0006）。前肢乳腺素（P-ACM / 0.0008）中，孕酮浓度显着较高，并显示出肺炎。乳腺雌激素和雌二醇浓度没有绝经显着差异，但绝经后女性乳房的乳腺浓度较高（p = 0.026和p-adj = 0.208）。乳腺雄激素与乳房密度明显相关（Spearman r = 0.27，P-adj = 0.02对于睾酮）和CMI（Spearman r = 0.3，P-adj = 0.038用于androstenione）。我们的数据表明，未来对乳腺类固醇激素的未来更大的研究以及其他参数是可行的。乳腺雄激素浓度与乳腺密度和基因甲基化的重要关联权证未来研究。 （c）2018年AACR。

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  • 来源
    《Cancer prevention research.》 |2018年第9期|共12页
  • 作者

    Lee Oukseub; Heinz Richard E.; Ivancic David; Muzzio Miguel; Chatterton Robert T.; Zalles Carola M.; Keeney Kara; Phan Belinda; Liu Dachao; Scholtens Denise; Fackler Mary Jo; Stearns Vered; Sukumar Saraswati; Khan Seema A.;

  • 作者单位

    Northwestern Univ Dept Surg Feinberg Sch Med Chicago IL 60611 USA;

    Northwestern Univ Dept Surg Feinberg Sch Med Chicago IL 60611 USA;

    Northwestern Univ Dept Surg Feinberg Sch Med Chicago IL 60611 USA;

    IIT Res Inst Chicago IL 60616 USA;

    Northwestern Univ Dept Obstet &

    Gynecol Feinberg Sch Med Chicago IL 60611 USA;

    Mercy Hosp Miami FL USA;

    Northwestern Univ Dept Surg Feinberg Sch Med Chicago IL 60611 USA;

    Northwestern Univ Dept Surg Feinberg Sch Med Chicago IL 60611 USA;

    Northwestern Univ Dept Surg Feinberg Sch Med Chicago IL 60611 USA;

    Northwestern Univ Prevent Med Chicago IL 60611 USA;

    Johns Hopkins Univ Sch Med Sidney Kimmel Comprehens Canc Ctr Baltimore MD USA;

    Johns Hopkins Univ Sch Med Sidney Kimmel Comprehens Canc Ctr Baltimore MD USA;

    Johns Hopkins Univ Sch Med Sidney Kimmel Comprehens Canc Ctr Baltimore MD USA;

    Northwestern Univ Dept Surg Feinberg Sch Med Chicago IL 60611 USA;

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  • 正文语种 eng
  • 中图分类 肿瘤学;
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