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首页> 外文期刊>Cancer prevention research. >Predicting the Presence of Oral Squamous Cell Carcinoma Using Commonly Dysregulated MicroRNA in Oral Swirls
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Predicting the Presence of Oral Squamous Cell Carcinoma Using Commonly Dysregulated MicroRNA in Oral Swirls

机译：在口腔漩涡中预测使用常见失去的微小RNA的口腔鳞状细胞癌存在

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摘要

Oral swirls are a noninvasive, rapidly collected source of salivary microRNA (miRNA) potentially useful in the early detection of disease states, particularly oral squamous cell carcinoma (OSCC). The aim of this study was to predict the presence of OSCC using a panel of OSCC-related dysregulated miRNA found in oral swirls, identified jointly in data from formalin-fixed paraffin-embedded (FFPE) and fresh-frozen specimens. Next-generation sequencing (NGS) was used to determine miRNA fold changes in FFPE OSCC specimens relative to histologically normal epithelium. These data were placed with NGS of fresh-frozen tissue data of The Cancer Genome Atlas database to select a panel of commonly dysregulated miRNA. This panel was then analyzed by RT-qPCR in RNA extracted from oral swirls collected from 30 patients with OSCC and 30 controls. Upregulation of miR-31 and miR-21 and downregulation of miR-99a, let-7c, miR-125b, and miR-100 were found between OSCC and controls in both FFPE and fresh-frozen samples. These miRNAs were studied in a training set of 15 OSCC versus 15 control oral swirls to develop a dysregulation score [AUC, 0.95; 95% confidence interval (CI), 0.88-1.03] and classification tree. A test cohort of 15 OSCC versus 15 control oral swirls yielded a dysregulation score AUC of 0.86 (95% CI, 0.79-1.00) with the classification tree identifying 100% (15/15) of OSCC and 67% (10/15) of controls. This study debuts the use of OSCC-associated miRNA, commonly dysregulated in both FFPE and frozen specimens, in oral swirls to indicate the presence of OSCC with high accuracy. (C) 2018 AACR.
机译：口腔漩涡是一种非侵入性的，迅速收集的唾液细胞源（miRNA）可能在早期检测疾病状态，特别是口腔鳞状细胞癌（OSCC）中有用。本研究的目的是使用在口腔漩涡中发现的OSCC相关的失败的miRNA面板预测OSCC的存在，该组织与福尔马林固定的石蜡包埋（FFPE）和新鲜冷冻标本共同鉴定。下一代测序（NGS）用于确定相对于组织学常规上皮的FFPE OSCC样本中的miRNA倍换。将这些数据与癌症基因组Atlas数据库的NGS的新鲜冷冻组织数据放置，以选择常见的miRNA的面板。然后通过从30例OSCC和30次对照组收集的口腔漩涡中提取的RTNA中的RTNA中的RTNA分析该面板。 MiR-31和miR-21的上调和miR-99a，Let-7c，miR-125b和miR-100的下调在FFPE和新鲜冷冻样品中的对照中发现。在15个OSCC的训练组中研究了这些miRNA，而15个控制口腔漩涡的训练套，以发展失效评分[AUC，0.95; 95％置信区间（CI），0.88-1.03]和分类树。 15个OSCC与15个控制口腔漩涡的测试队列产生了0.86（95％CI，0.79-1.00）的失效评分AUC，分类树识别OSCC的100％（15/15）和67％（10/15）控制。本研究首次揭示使用OSCC相关的miRNA，在口腔漩涡中常见于FFPE和冷冻标本中的使用，以指示高精度的OSCC。 （c）2018年AACR。

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  • 来源
    《Cancer prevention research.》 |2018年第8期|共11页
  • 作者

    Yap Tami; Koo Kendrick; Cheng Lesley; Vella Laura J.; Hill Andrew F.; Reynolds Eric; Nastri Alf; Cirillo Nicola; Seers Christine; McCullough Michael;

  • 作者单位

    Univ Melbourne Melbourne Dent Sch Melbourne Vic Australia;

    Royal Melbourne Hosp Dept Surg Melbourne Vic Australia;

    La Trobe Univ Dept Biochem &

    Genet La Trobe Inst Mol Sci Bundoora Vic Australia;

    Florey Inst Neurosci &

    Mental Hlth Parkville Vic Australia;

    La Trobe Univ Dept Biochem &

    Genet La Trobe Inst Mol Sci Bundoora Vic Australia;

    Univ Melbourne Melbourne Dent Sch Melbourne Vic Australia;

    Royal Melbourne Hosp Dept Oral &

    Maxillofacial Surg Melbourne Vic Australia;

    Univ Melbourne Melbourne Dent Sch Melbourne Vic Australia;

    Univ Melbourne Melbourne Dent Sch Melbourne Vic Australia;

    Univ Melbourne Melbourne Dent Sch Melbourne Vic Australia;

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  • 正文语种 eng
  • 中图分类 肿瘤学;
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