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首页> 外文期刊>Catalysis Today >Non-covalent and covalent immobilization of Candida antarctica lipase B on chemically modified multiwalled carbon nanotubes for a green acylation process in supercritical CO2
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Non-covalent and covalent immobilization of Candida antarctica lipase B on chemically modified multiwalled carbon nanotubes for a green acylation process in supercritical CO2

机译：在超临界CO2中对化学改性多晶碳纳米管的非共价和共价固定化学改性多壁碳纳米管中的用于超临界CO2中的绿色酰化过程

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摘要

Candida antarctica B lipase (CAL-B) was immobilized on purified and functionalized multiwalled carbon nanotubes (MWCNTs). Both immobilization routes, physical adsorption and covalent bonding, were investigated. MWCNT functionalization by a non-aggressive oxidation by potassium permanganate led to an interesting balance between the hydrophilic and the hydrophobic areas of the MWCNT surface; the former being responsible of the good dispersion of MWCNTs in water and the latter having a favorable affinity with CAL-B. The enzyme loadings reached were significant: around 16 wt. % and 21 wt.% for non-covalent and covalent immobilization, respectively. The enzymatic activity was studied with the reaction of O-acylation of geraniol into geranyl acetate by CAL-B in supercritical CO2. Even if a decay in synthesis of geranyl acetate was observed over cycling for both CAL-B@MWCNT catalysts, it was demonstrated that the regioselectivity of CAL-B was unchanged through immobilization on the MWCNT surface for both routes. Interestingly, it was shown that a fully green enzymatic process can be achieved with these prepared CAL-B@MWCNT biocatalyst. Such approach could be transferred to other support/enzyme systems for developing new eco-friendly synthesis processes.
机译：将Candida抗钙CAILCA（CAL-B）固定在纯化和官能化的多壁碳纳米管（MWCNT）上。研究了固定途径，物理吸附和共价键合。 MWCNT通过高锰酸钾的非侵蚀性氧化官能化导致MWCNT表面的亲水和疏水区域之间的有趣平衡;前者负责MWCNTS在水中的良好分散，后者与Cal-B具有有利亲和力。达到的酶载体显着：大约16wt。 ％和21重量％分别用于非共价和共价固定。通过CAL-B在超临界CO2中通过将Geraniol的O-酰化成乙酸乙酸甲酯反应进行酶活性。即使在对Cal-B @ MWCNT催化剂的循环上观察到乙酸甲酯的合成的衰减，也证明Cal-B的区域选择性通过固定在MWCNT表面上的用于两条途径而保持平静。有趣的是，结果表明，通过这些制备的Cal-B @ MWCNT生物催化剂可以实现全绿色酶促方法。这种方法可以转移到其他支持/酶系统中，以开发新的环保合成过程。

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