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首页> 外文期刊>Cell motility and the cytoskeleton >Microtubule plus-end and minus-end capture at adherens junctions is involved in the assembly of apico-basal arrays in polarised epithelial cells.
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Microtubule plus-end and minus-end capture at adherens junctions is involved in the assembly of apico-basal arrays in polarised epithelial cells.

机译：粘附结的微管加端和减去末端捕获参与偏振上皮细胞的Apico-Basal阵列的组装。

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Apico-basal polarisation of epithelial cells involves a dramatic reorganisation of the microtubule cytoskeleton. The classic radial array of microtubules focused on a centrally located centrosome typical of many animal cells is lost or greatly reduced and a non-centrosomal apico-basal array develops. The molecules and mechanisms responsible for the assembly and positioning of these non-centrosomal microtubules have not been fully elucidated. Using a Nocodazole induced regrowth assay in invitro culture (MDCK) and in situ epithelial (cochlear Kolliker's) cell models we establish that the apico-basal array originates from the centrosome and that the non-centrosomal microtubule minus-end anchoring sites do not contribute significantly to their nucleation. Confocal and electron microscopy revealed that an extended radial array assembles with microtubule plus-ends targeting cadheren sites at adherens junctions and EB1 and CLIP-170 co-localising with beta-catenin and dynein clusters at the junction sites. The extended radial array is likely to be a vital intermediate step in the assembly process with cortical anchored dynein providing the mechanical force required for microtubule release, translocation and capture. Ultrastructural analyses of the apico-basal arrays in fully polarised MDCK and Kolliker's cells revealed microtubule minus-end association with the most apical adherens junction (Zonula adherens). We propose that a release and capture model involving both microtubule plus- and minus-end capture at adherens junctions is responsible for the generation of non-centrosomal apico-basal arrays in most centrosome containing polarised epithelial cells.
机译：上皮细胞的APICO-基础偏振涉及微管细胞骨架的显着重组。聚焦在典型的许多动物细胞的中心位于中心位位于许多动物细胞的中心的经典径向阵列被丢失或大大降低，并且产生非Centsomal Apico-Basal阵列。负责这些非中心微管的组装和定位的分子和机制尚未完全阐明。使用Nocodazole在invitro培养（mdck）和原位上皮（耳蜗Kolliker）细胞模型中，我们建立了Apico-base阵列源自中心体，并且非Centrosomal Microotubule负端锚定部位没有显着贡献他们的成核。共聚焦和电子显微镜揭示了一种延长的径向阵列与微管加上靶向粘附结和EB1和夹子-170的粘附位点，并在结位点的β-catenin和Dynein簇共定。延长的径向阵列可能是组装过程中的重要中间步骤，其具有皮质锚定量，提供微管释放，易位和捕获所需的机械力。完全偏振MDCK和Kolliker细胞中APICO基础阵列的超微结构分析揭示了与最顶端粘附结（Zonula angerens）的微管负关联。我们提出涉及在粘附结处的微管和负端捕获的释放和捕获模型是负责在大多数含离心体上皮细胞中产生非Centsomal Apico-Basal阵列的原因。

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