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首页> 外文期刊>Cell motility and the cytoskeleton >Mammalian Navigators are microtubule plus-end tracking proteins that can reorganize the cytoskeleton to induce neurite-like extensions.
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Mammalian Navigators are microtubule plus-end tracking proteins that can reorganize the cytoskeleton to induce neurite-like extensions.

机译：哺乳动物导航员是微管加端跟踪蛋白，可以重新组织细胞骨架以诱导神经沸石的延伸。

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摘要

Mammalian microtubule plus-end tracking proteins (+TIPs) specifically associate with the ends of growing microtubules. +TIPs are involved in many cellular processes, including mitosis, cell migration and neurite extension. Navigators are mammalian homologues of the C. elegans unc-53 protein, an ATPase that has been linked to the migration and outgrowth of muscles, axons and excretory canals. Here we show that all three mammalian Navigators are +TIPs, consistent with a previous study on Navigator 1 (NAV1) (Martinez-Lopez et al., Mol Cell Neurosci 2005;28:599-612). Overexpression of GFP-tagged Navigators causes displacement of CAP_GLY-motif containing +TIPs, such as CLIP-170, from microtubule ends, suggesting that the Navigator-binding sites on microtubule ends overlap with those of the CAP_GLY-motif proteins. In interphase cells, mammalian Navigators also prominently localize to centrosomes, a localization that does not depend on an intact microtubule network. Fluorescence recovery after photobleaching (FRAP) experiments indicate that NAV1 associates with intracellular structures other than microtubules or centrosomes. Expression of GFP-tagged Navigators induces the formation of neurite-like extensions in non-neuronal cells, showing that Navigators can dominantly alter cytoskeletal behavior. For NAV1 this function depends on its ATPase activity; it is not achieved by a classical type of MT bundling and stabilization. Combined our data suggest that Navigators are +TIPs that can reorganize the cytoskeleton to guide cell shape changes. Our data are consistent with a role for Navigators in neurite outgrowth.
机译：哺乳动物微管加端跟踪蛋白（+尖端）与生长微管的末端有关。 +提示涉及许多细胞过程，包括有丝分裂，细胞迁移和神经突延伸。导航员是C.秀丽隐杆线虫UNC-53蛋白的哺乳动物的同源物，这是一种与肌肉，轴突和排泄运河的迁移和生长相关的ATP酶。在这里，我们表明所有三个哺乳动物导航员都是+提示，与前一对导航器1的研究一致（NAV1）（Martinez-Lopez等，Mol Cell Neurosci 2005; 28：599-612）。 GFP标记导航仪的过度表达导致含Cap_Gly-MOTIF的含量+提示，例如夹子-170，例如夹子-170，从微管末端表明MicroTumule末端的导航仪结合位点与Cap_gly-motif蛋白的导航末端重叠。在相互间细胞中，哺乳动物导航员也突出地定位为Centrosomes，一种不依赖于完整的微管网络的本地化。光漂白（FRAP）实验后的荧光恢复表明NAV1与微管或中心素以外的细胞内结构相关联。 GFP标记导航仪的表达诱导非神经元细胞中的神经沸石延伸的形成，显示导航仪可以显着改变细胞骨骼行为。对于NAV1，此功能取决于其ATPASE活动;它不是通过经典类型的MT捆绑和稳定来实现。组合我们的数据表明导航器是+尖端，可以重新组织细胞骨架以引导细胞形状变化。我们的数据与Neureite Forgrowth中的导航员的作用一致。

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