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Phenotypic stability of the human MG-63 osteoblastic cell line at different passages

机译:不同通道的人Mg-63骨细胞细胞系的表型稳定性

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摘要

One of the most popular cell lines in osteogenesis studies is the human osteoblastic line MG-63. For cell biological investigation, it is important that the cells remain stable in their phenotype over several passages in cell culture. MG-63 cells can be used to provide fundamental insights into cell--material interaction. The aim of this study is to present a systematic characterization of the physiological behavior of MG-63 cells in the range of passages 5-30. Significant cell physiology processes during the first 24 h, including cell morphology, availability of adhesion receptors, cell cycle phases, as well as the expression of the signaling proteins Akt, GSK3a/b, IkB-alpha, ERK1/2, p38-MAPK, and intracellular calcium ion mobilization, remained stable over the entire range of passages P5-P30. Due to these stable characteristics in a wide range of cell culture passages, MG-63 cells can be considered as a suitable in vitro model to analyze the biocompatibility and biofunctionality of implant materials.
机译:成骨研究中最受欢迎的细胞系之一是人骨细胞线MG-63。对于细胞生物学调查,重要的是细胞在其细胞培养的几个通道中在其表型中保持稳定。 Mg-63细胞可用于提供对细胞材料相互作用的根本洞察。本研究的目的是在通道5-30的范围内呈现MG-63细胞的生理行为的系统表征。在前24小时期间的显着细胞生理过程,包括细胞形态,粘附受体的可用性,细胞周期阶段,以及信号传导蛋白Akt,GSK3A / B,IKB-α,ERK1 / 2,P38-MAPK的表达,和细胞内钙离子摩擦,在整个通道P5-P30范围内保持稳定。由于这些稳定的细胞培养通道中的特性,Mg-63细胞可以被认为是分析植入物材料的生物相容性和生物相容性的合适的体外模型。

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