• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >MicroRNA‐1204 promotes cell proliferation by regulating PITX1 in non‐small‐cell lung cancer
【24h】

MicroRNA‐1204 promotes cell proliferation by regulating PITX1 in non‐small‐cell lung cancer

机译：MicroRNA-1204通过在非小细胞肺癌中调节PITX1来促进细胞增殖

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract MicroRNA‐1204 (miR‐1204), a member of the PVT1 region, may improve B cell differentiation and metastasis in breast cancer. However, the role of miR‐1204 in non‐small‐cell lung cancer (NSCLC) and its mechanism remain unclear. The GEO public database was first employed to find differentially expressed genes. The expression level of miR‐1204 in patient tissues and NSCLC cell lines was determined using qRT‐PCR. Cell proliferation assays were performed to investigate the impact of miR‐1204 on cell growth. Bioinformatics analysis and dual‐luciferase reporter assays were conducted to find potential target genes. Finally, we performed in vivo experiments to identify the effect of miR‐1204 on tumor formation in nude mice. It was first found that miR‐1204 was overexpressed in NSCLC tissues and cells. miR‐1204 increased the proliferation of NSCLC cells and reduced cell cycle arrest in vitro. PITX1 (paired like homeodomain 1) was found as a potential target gene. In addition, PITX1 was also found to be low in expression in NSCLC tissues and cells. To show that PITX1 reversed the function of miR‐1204 in promoting proliferation, confirmatory experiments were performed. Moreover, high miR‐1204 and low PITX1 expression was highly correlated with tumor size, lymph node metastasis, and the TNM stage in patients diagnosed with NSCLC. Our results suggested that upregulated miR‐1204 in NSCLC is associated with NSCLC progression and promotes NSCLC cell proliferation by downregulating PITX1. miR‐1204 may act as a poor prognostic factor and a potential therapeutic target for NSCLC.
机译：摘要MicroRNA-1204（MIR-1204），PVT1区域的成员，可以改善乳腺癌中的B细胞分化和转移。然而，MIR-1204在非小细胞肺癌（NSCLC）中的作用及其机制仍不清楚。首次采用Geo公共数据库来查找差异表达的基因。使用QRT-PCR测定患者​​组织和NSCLC细胞系中miR-1204的表达水平。进行细胞增殖测定以研究miR-1204对细胞生长的影响。进行生物信息学分析和双荧光素酶报告分析以找到潜在的靶基因。最后，我们在体内实验中进行，以鉴定miR-1204对裸鼠肿瘤形成的影响。首先发现MIR-1204在NSCLC组织和细胞中过表达。 miR-1204增加了Nsclc细胞的增殖，并在体外降低细胞周期捕获。发现PitX1（像同源域1配对）作为潜在的靶基因。另外，在NSCLC组织和细胞中也发现PITX1在表达中。为了表明PITX1反转miR-1204在促进增殖方面的功能，进行了确认实验。此外，高miR-1204和低pitx1表达与肿瘤大小，淋巴结转移和诊断NSCLC患者的TNM阶段高度相关。我们的结果表明，NSCLC中的上调MIR-1204与NSCLC进展相关，并通过下调PITX1促进NSCLC细胞增殖。 MiR-1204可以作为NSCLC的预后因子和潜在治疗靶标。

著录项

  • 来源
    《Cell biology international.》 |2019年第3期|共12页
  • 作者

    Jiang Wei; He Yaozhou; Shi Yijun; Guo Zizhang; Yang Shikun; Wei Ke; Pan Chunfeng; Xia Yang; Chen Yijiang;

  • 作者单位

    Department of Thoracic and Cardiovascular SurgeryThe First Affiliated Hospital of Nanjing Medical;

    Department of Thoracic and Cardiovascular SurgeryThe First Affiliated Hospital of Nanjing Medical;

    Department of Thoracic and Cardiovascular SurgeryThe First Affiliated Hospital of Nanjing Medical;

    Department of Thoracic and Cardiovascular SurgeryThe First Affiliated Hospital of Nanjing Medical;

    Department of Hepatobiliary/Liver Transplantation CenterThe First Affiliated Hospital of Nanjing;

    Department of Thoracic and Cardiovascular SurgeryThe First Affiliated Hospital of Nanjing Medical;

    Department of Thoracic and Cardiovascular SurgeryThe First Affiliated Hospital of Nanjing Medical;

    Department of Thoracic and Cardiovascular SurgeryThe First Affiliated Hospital of Nanjing Medical;

    Department of Thoracic and Cardiovascular SurgeryThe First Affiliated Hospital of Nanjing Medical;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    microRNA‐1204; non‐small‐cell lung cancer; PITX1; proliferation;

    机译：microRNA-1204;非小细胞肺癌;PITX1;增殖;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号