• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >The different expression of key markers on urothelial holoclonal, meroclonal, and paraclonal cells in in vitro culture
【24h】

The different expression of key markers on urothelial holoclonal, meroclonal, and paraclonal cells in in vitro culture

机译：在体外培养中尿路上全伦，塞洛克族，乳头族和帕拉克隆细胞的关键标志物的不同表达

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Urothelial cell populations which differ in morphology and proliferation capacities can be isolated from the urinary bladder. The goal of this study was to analyze a clonal, proliferative, and self‐renewing potential of porcine urothelial cells and to compare expression of selected adhesion and tight junction molecules, urothelial and stem cell markers for the urothelial clone types. Urothelial cells were isolated from 10 porcine urinary bladders. Three different clone types: holoclone‐, meroclone‐and paraclone‐like colonies were identified based on their morphology. To characterize and compare the urothelial clones the immunofluorescent stains were performed. Expression of pancytokeratin (PanCK), Ki‐67 and p63 was higher for holoclone‐ like cells compared to meroclone‐and paraclone‐like cells ( P? ?0.05). Meroclone‐like cells expressed higher levels of p63 compared to paraclone‐ like cells ( P? ?0.05). The level of Ki‐67 and PanCK for meroclone‐ and paraclone‐ like cells was comparable ( P? ?0.05). β1 and β4 integrins were not expressed. Expression of zonula occludens‐1 (ZO‐1) in cell‐cell junctions for paraclone‐, meroclone‐and holoclone‐like cells was 17.6?±?0.6, 14.7?±?0.5, and 16.1?±?0.4, respectively. The results of actin filaments (F‐actin) expression were 253,634?±?6,920 for meroclone‐like cells, 198,512?±?7,977 for paraclone‐like cells and 133,544?±?3,169 for holoclone‐like cells. Three urothelial cell types with differing features can be isolated from the bladder. Holoclone‐like cells are the richest in stem cells and should be used in further studies for construction of neo‐bladder or neo‐conduit using tissue engineering methods.
机译：抽象的尿路上皮细胞群不同于形态和增殖能力，可以从膀胱中分离出来。本研究的目的是分析猪尿路皮细胞的克隆，增殖和自我更新潜力，并比较尿路上克隆类型的选定粘附和紧密结分子，尿路上皮和干细胞标志物的表达。从10个猪尿的膀胱中分离尿路皮细胞。三种不同的克隆类型：基于它们的形态来鉴定Holoclone，Meroclone-and和帕拉环甾酮样菌落。表征和比较尿液克隆，进行了免疫荧光污渍。与梅罗仑和帕拉环甾酮样细胞相比，HoloClone样细胞的表达，Ki-67和P63的表达更高与Paraclone样细胞相比，Meroclone样细胞表达了更高水平的p63（p≤≤0.05）。 Ki-67和帕拉卡酮和帕拉卡酮样细胞的平板水平相当（p？＆ 0.05）。没有表达β1和β4整联蛋白。唑醇 - ，柏拉酮 - 和全洛冬细胞的细胞 - 细胞连接中的zonula occludens-1（zo-1）的表达分别为17.6Ω·α0.6,14.7°+ 0.4。肌动蛋白长丝（F-actin）表达的结果为柏拉酮样细胞的253,634±6,920℃，198,512〜±7,977个用于帕拉酮醇样细胞，133,544〜±3,169用于全隆的细胞。可以从膀胱中分离具有不同特征的三种尿路上皮细胞类型。 HoloClone样细胞是干细胞中最富含的干细胞，并且应该在进一步的研究中使用组织工程方法构建新膀胱或新导管。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号