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Quantitative analysis on secretion level of CDNF regulated by two key alpha-helices in CDNF protein

机译：CDNF蛋白两种关键α-螺旋调节CDNF分泌水平的定量分析

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Cerebral dopamine neurotrophic factor (CDNF) has been considered as potent candidates for the therapy of Parkinson's disease (PD) for which can promote the survival of midbrain dopaminergic neurons. In addition to secret out from cells like other classical neurotrophic factors (NTFs), CDNF can locate in the endoplasmatic reticulum (ER), where they can function as ER stress response protein to regulate ER stress. In our previous studies, we have found two helices, alpha 1 and alpha 7, which can regulate the intracellular trafficking and secretion of CDNF. alpha 1 distruction can significantly retain CDNF protein in the ER, but alpha 7 distruction induce most CDNF protein secreting out the cells. Then alpha 1 and alpha 7 regulate protein trafficking and secretion in opposite side. However, the exact secretion level of CDNF affected by alpha 1 or alpha 7 have not been sensitively quantified. In this study, we used nanoluciferase to quantify the secretion level of CDNF protein so that we could evaluate the impact of alpha 1 and alpha 7 on CDNF secretion or function.
机译：脑多巴胺神经营养因子（CDNF）被认为是帕金森病（PD）治疗的有效候选者，其可以促进中脑多巴胺能神经元的存活。除了从类似于其他经典神经营养因子（NTFS）的细胞中秘密外，CDNF可以定位在内粒状网状物（ER）中，它们可以用作ER应激反应蛋白来调节ER应激。在我们以前的研究中，我们发现了两个螺旋，α1和α7，可以调节CDNF的细胞内贩运和分泌。 α1分类可以显着保留在ER中的CDNF蛋白，但α7分类诱导大多数CDNF蛋白分泌细胞。然后α1和α7调节对面的蛋白质贩运和分泌。然而，受α1或α7影响的CDNF的确切分泌水平尚未敏感地量化。在该研究中，我们使用纳米琥珀酶量化CDNF蛋白的分泌水平，以便我们可以评估α1和α7对CDNF分泌或功能的影响。

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