• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Let‐7b‐5p inhibits proliferation and motility in squamous cell carcinoma cells through negative modulation of KIAA1377
【24h】

Let‐7b‐5p inhibits proliferation and motility in squamous cell carcinoma cells through negative modulation of KIAA1377

机译：Let-7B-5P通过KIAA1377的阴性调节抑制鳞状细胞癌细胞中的增殖和运动

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract KIAA1377 has been found to be linked with lymph node metastasis in esophageal squamous cell carcinoma (SCC) in our previous study; however, the regulation of KIAA1377 remains far from understood. Herein, to understand the regulation of KIAA1377 from the angle of microRNA (miRNA)–messenger RNA (mRNA) modulation in the setting of SCC cells, the basal level of KIAA1377 was determined by quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blot analysis in KYSE‐150 and HeLa cells; biological roles of KIAA1377 contributing in the proliferation, migration, and invasion were evaluated using 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT), wound‐healing and Transwell assays, respectively, after KIAA1377 was knocked out mediated by the CRISPR‐Cas9 system. Bioinformatic prediction revealed that let‐7b‐5p was a putative miRNA regulating KIAA1377, which was ensuingly validated by the luciferase reporter assay; after which, variation of KIAA1377 expression was further verified by qRT‐PCR and western blot analysis. Moreover, the biological roles of let‐7b‐5p in proliferation, migration, and invasion of KYSE‐150 and HeLa cells were also evaluated. It was exhibited that KIAA1377 was able to promote the proliferation and motility of both KYSE‐150 and HeLa cells, which can be reverted by re‐expression of let‐7b‐5p. The luciferase reporter assay verified that let‐7b‐5p can diametrically target KIAA1377. Collectively, our data demonstrated that let‐7b‐5p can directly but negatively regulate KIAA1377 in SCC cell lines, Ecal109, and HeLa cells.
机译：在我们以前的研究中发现摘要KIAA1377与食管鳞状细胞癌（SCC）中的淋巴结转移相关联;然而，KiaA1377的监管仍然远未理解。在此，为了从微小RORNA（miRNA）-messenger RNA（mRNA）调节在SCC细胞的凝固中，理解KIAA1377的调节，通过定量的实时聚合酶链反应（QRT-PCR）测定KIAA1377的基础水平Kyse-150和HeLa细胞中的Western印迹分析;使用3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2H-四唑溴铵（MTT），伤口愈合和Transwell测定评估KiaA1377在增殖，迁移和侵袭中产生的增殖，迁移和侵袭的生物学作用。分别在KIAA1377被CRISPR-CAS9系统介导的KIAA1377之后。生物信息预测显示，Let-7B-5P是调节KIAA1377的推定miRNA，其被荧光素酶报告结果测定继续验证;此后，通过QRT-PCR和Western印迹分析进一步验证KIAA1377表达的变化。此外，还评估了Kyse-150和HeLa细胞增殖，迁移和侵袭的Let-7b-5p的生物学作用。展示了KiaA1377能够促进Kyse-150和HeLa细胞的增殖和动力，可以通过重新表达Let-7b-5p来再转染。荧光素酶报告器测定验证了Let-7B-5P可以直截了当地靶向KiaA1377。集体，我们的数据表明，Let-7B-5P可以直接但在SCC细胞系，Ecal109和HeLa细胞中直接调节KiaA1377。

著录项

  • 来源
    《Cell biology international.》 |2019年第6期|共8页
  • 作者

    Zheng Shutao; Liu Qing; Ma Rong; Tan Doudou; Shen Tongxue; Zhang Xiao; Lu Xiaomei;

  • 作者单位

    Clinical Medical Research InstituteFirst Affiliated Hospital of Xinjiang Medical UniversityXinjiang;

    Clinical Medical Research InstituteFirst Affiliated Hospital of Xinjiang Medical UniversityXinjiang;

    Tumor Hospital Affiliated to Xinjiang Medical UniversityXinjiang Uygur Autonomous Region Urumqi;

    Tumor Hospital Affiliated to Xinjiang Medical UniversityXinjiang Uygur Autonomous Region Urumqi;

    Tumor Hospital Affiliated to Xinjiang Medical UniversityXinjiang Uygur Autonomous Region Urumqi;

    Tumor Hospital Affiliated to Xinjiang Medical UniversityXinjiang Uygur Autonomous Region Urumqi;

    Clinical Medical Research InstituteFirst Affiliated Hospital of Xinjiang Medical UniversityXinjiang;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    CRISPR‐Cas9; KIAA1377; let‐7b‐5p; motility; squamous cell carcinoma;

    机译：CRISPR-CAS9;KIAA1377;LET-7B-5P;运动;鳞状细胞癌;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号