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首页> 外文期刊>Cell biology international. >Different levels of testicular organization during gonadal differentiation in B6.y tir mice manifesting sex reversal
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Different levels of testicular organization during gonadal differentiation in B6.y tir mice manifesting sex reversal

机译：在B6.Y TIR小鼠中的性别逆转期间，在B6.Y小鼠中的睾丸组织不同水平的睾丸组织

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B6.Y Tir (mice with Y chromosome from a strain in Tirano, Italy, and autosomes and X-chromosomes from the B6 strain) mice provide an excellent model for analysing sex development that occurs during gonadal differentiation; however, the molecular mechanisms that contribute to sex reversal are unclear. Our aim has been to establish which molecular events participate in this sex reversal. The pattern of gene expression related to testicular [Sry (sex-determining region of the Y chromosome), Sox9 (Sry-related high-mobility group box gene 9) and Mis (Mü llerian-inhibiting substance)] and ovarian [Wnt4 (Wingless-type MMTV (murine-mammary-tumour virus) integration site family, member 4), Rspo1 (cysteine-rich secretory protein containing a thrombospondin type 1 repeat) and Stra8 (stimulated by retinoic acid gene 8)] differentiation was analysed by applying immunofluorescence and real-time RT-PCR (reverse transcription-PCR), focusing on XY gonads from the B6.Y Tir mouse, but also analysing the normal strains CD-1 and C57BL/6J (B6). The expression of genes related to the process of sexual differentiation was altered in the case of the B6.Y Tir strain, both at the transcript and protein level, inducing differentiation of ovaries and ovotestes, but not the formation of the testes, which were normal. Our results indicate that the expression of testicular genes is inhibited at various levels, permitting the expression of ovarian genes such as Wnt4, Stra8 and Rspo1. However, their activity was not clear when the data were averaged. Correlation analysis indicated that an ovary differentiation pathway is activated when the testicular differentiation pathway is inhibited.
机译：B6.Y TIR（来自蒂拉诺，意大利，意大利的菌株的Y染色体和来自B6菌株的X染色体的小鼠）小鼠提供了一种优异的模型，用于分析性能发展期间的性能;然而，有助于性逆转的分子机制尚不清楚。我们的目标是建立哪些分子事件参与这种性行为逆转。与睾丸相关的基因表达模式[Sry（y染色体的性别测定区域），SOX9（SRY相关高迁移率组箱基因9）和MIS（Müllerian抑制物质）]和卵巢[Wnt4（无翼 - 通过施加免疫荧光分析通过施加免疫荧光来分析分析的 - 型MMTV（尿蛋白 - 肿瘤病毒）集成位点系列系列，成员4）和STRA8（通过视黄酸基因8）分析的分化和实时RT-PCR（逆转录PCR），聚焦来自B6.Y TIR小鼠的XY GONAD，还聚在常规菌株CD-1和C57BL / 6J（B6）中分析。在B6.Y TIR菌株的情况下，在转录物和蛋白质水平的情况下，改变了与性分化过程的基因的表达被改变，诱导卵巢和Ovotestes的分化，但不是正常的睾丸的形成。我们的结果表明，睾丸基因的表达被抑制在各种水平，允许卵巢基因如Wnt4，STRA8和RSPO1表达。但是，当数据平均时，它们的活动尚不清楚。相关分析表明，当抑制睾丸分化途径时，卵巢分化途径被激活。

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