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首页> 外文期刊>Cell biology international. >A new approach for pancreatic tissue engineering: human endometrial stem cells encapsulated in fibrin gel can differentiate to pancreatic islet beta-cell
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A new approach for pancreatic tissue engineering: human endometrial stem cells encapsulated in fibrin gel can differentiate to pancreatic islet beta-cell

机译：胰组织工程的一种新方法：包封在纤维蛋白凝胶中的人子宫内膜干细胞可以分化为胰岛β细胞

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摘要

Metabolic diabetes mellitus as the most serious and prevalent metabolic disease in the world has various complications. The most effective treatment of type I diabetes seems to be islet cell transplantation. Shortage of donors and difficult procedures and high rate of rejection have always restricted this approach. Tissue engineering is a novel effective solution to many medical problems such as diabetes. Endometrial mesenchymal stem cells as a lineage which have the potential to differentiate to mesodermal and endodermal tissues seem to be suitable for this purpose. Fibrin hydrogel with a high degree of biocompatibility and specific properties making it similar to normal pancreas seems to be an ideal scaffold. After successfully isolating stem cells (hEnSCs) from human endometrium, a three-step protocol was used to differentiate them into pancreatic beta cells. Fibrin was used as 3D scaffold. After 2 weeks, cells formed clusters like islets cells, and secretion of insulin was measured by chemiluminescence. PDX1, proinsulin, and c-peptide as special markers of beta cells were detected by immunofiuorescence. Expression of glucagon, PDX1, and insulin genes in mRNA level was detected by Real time PCR and gel electrophoresis. The former showed higher levels of gene expression in 3D cultures. SEM analysis showed good integrity between cells and scaffold. No toxicity was detected with fibrin scaffold by MTT assay.
机译：代谢糖尿病Mellitus作为世界上最严重和最普遍的代谢疾病具有各种并发症。 I型糖尿病最有效的治疗似乎是胰岛细胞移植。捐助者短缺和困难程序和高拒绝率始终限制了这种方法。组织工程是一种新的有效解决方案，对糖尿病等许多医疗问题。子宫内膜间充质干细胞作为一种谱系，其具有区分与中胚层和内胚组织的潜在似乎适用于此目的。具有高生物相容性和特定性质的纤维蛋白水凝胶使其类似于正常胰腺似乎是理想的支架。在成功地将干细胞（Henscs）与人子宫内膜分离后，使用三步方案将它们分化为胰腺β细胞。纤维蛋白用作3D支架。 2周后，细胞形成了胰岛细胞等簇，通过化学发光测量胰岛素的分泌。 PDX1，胰岛素和C-肽作为β细胞的特殊标志物被免疫荧光检测。通过实时PCR和凝胶电泳检测mRNA水平中胰高血糖素，PDX1和胰岛素基因的表达。前者在3D培养物中显示出更高水平的基因表达。 SEM分析显示细胞和支架之间的良好完整性。通过MTT测定法用纤维蛋白支架检测毒性。

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