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首页> 外文期刊>Cell biology international. >Comparative study of directional differentiation of human and mouse embryonic stem cells into cardiomyocytes
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Comparative study of directional differentiation of human and mouse embryonic stem cells into cardiomyocytes

机译：人参向胚胎干细胞定向分化对心肌细胞的比较研究

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This comparative study investigates the method, efficiency, and anti-hypoxic ability of cardiomyocytes, directionally induced from human (h) and mouse (m) embryonic stem cells (ESCs). hESCs were induced into cardiomyocytes by suspension culture, without inducers, or adherent culture using the inducers activin A and BMP4. mESCs were induced into cardiomyocytes by hanging-drop method, without inducers or induced with vitamin C. All four methods successfully induced ESCs to differentiate into cardiomyocytes. There was a significant difference between groups with and without inducers. A significant difference was found between mESC and hESC groups with inducers. The average beating frequency of cardiomyocytes differentiated from hESC was lower than cardiomyocytes differentiated from mESC, while the average beating frequency of cardiomyocytes differentiated from the same cell line, despite different culture methods, did not differ. Beating cardiomyocytes of each group were positive for cTnT staining. Spontaneous action potentials of beating cardiomyocytes were detected by patch-clamp experiments in each group. Different apoptotic ratios were detected in beating cardiomyocytes in each group and the difference between cardiomyocytes induced from mESCs and hESCs was statistically significant. The differentiation efficiencies in the groups without inducers were significantly higher than those without inducers. The induction of mESCs was more simple and efficient compared with hESCs. Without the presence of other protective factors, the anti-hypoxic ability of cardiomyocytes induced from hESCs was stronger and the beating times were longer in vitro compared with mESCs.
机译：该比较研究研究了心肌细胞的方法，效率和抗缺氧能力，定向从人（H）和小鼠（M）胚胎干细胞（ESC）诱导。使用诱导剂Activin A和BMP4，通过悬浮培养物，无诱导剂或粘附培养物诱导HESCY细胞。通过悬垂方法诱导MESCs诱导心肌细胞，没有诱导剂或用维生素C诱导。所有四种方法都成功地诱导了ESC分化为心肌细胞。有和没有诱导者的群体之间存在显着差异。 MESC和HESC群体与诱导者之间发现了显着差异。从HESC的分化的心肌细胞的平均跳动频率低于来自MESC的心肌细胞，而在不同细胞系中的心肌细胞的平均跳动频率尽管不同的培养方法，但没有差异。每组的击打心肌细胞对于CTNT染色是阳性的。通过每组的贴片钳实验检测打击心肌细胞的自发动作电位。在击打每组的心肌细胞时检测到不同的凋亡比，并且麦塞斯和HESC诱导的心肌细胞之间的差异是统计学意义。没有诱导剂的组中的分化效率显着高于没有诱导剂的效率。与HESC相比，MESCS的诱导更简单且有效。在没有其他保护因子的情况下，HESC诱导的心肌细胞的抗缺氧能力较强，与MESCS相比，体外搅拌时间较长。

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