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首页> 外文期刊>Cell biology international. >Gene expression profile analysis of dbpA knockdown in colorectal cancer cells
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Gene expression profile analysis of dbpA knockdown in colorectal cancer cells

机译：结直肠癌细胞DBPA敲低的基因表达谱分析

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摘要

DNA-binding protein A (dbpA) has been reported associated with the pathogenesis and development of various cancers. However, no evidence showed the gene expression profiling alternation involved in dbpA knockdown in colorectal cancer (CRC) cells. Small interference RNA (siRNA) method was used to knock down dbpA expression in SW620 cells. The changes of gene expression profiles in dbpA knockdown SW620 cells were determined by microarray analysis and Western blot. A total of 578 genes expressed differentially (twofold change), 181 genes were up-regulated, and 397 down-regulated in the dbpA knockdown group in comparison with the control group. The discrimination reliability was further verified by principal component analysis and Pearson correlation. Gene ontology (GO) pathway analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the most significantly expressed genes were linked to MAPK signaling pathway. Furthermore, Western blot verified that dbpA knockdown directly inhibited the activations of TAK1, p38, and JNK in CRC cells. In conclusion, dbpA knockdown in SW620 cells altered the expression of carcinogenesis-associated genes in CRC and the involvement of dbpA on CRC might through MAPK signaling pathway, which might provide valuable evidence to further investigate the correlation between dbpA expression and CRC development.
机译：已经报道了DNA结合蛋白A（DBPA）与各种癌症的发病机制和发育相关。然而，没有证据表明在结肠直肠癌（CRC）细胞中DBPA敲低涉及的基因表达分析交替。使用小干扰RNA（siRNA）方法在SW620细胞中击退DBPA表达。通过微阵列分析和Western印迹测定DBPA敲低SW620细胞中基因表达谱的变化。与对照组相比，共调节总共578个基因差异（双重变化），181个基因，并在DBPA敲低组中下调397。通过主成分分析和Pearson相关性进一步验证了歧视可靠性。基因本体（GO）途径分析和基因和基因组（Kegg）途径分析显示，最显着表达的基因与MAPK信号通路连接。此外，Western Blot验证了DBPA敲低直接抑制CRC细胞中TAK1，P38和JNK的激活。总之，SW620细胞中的DBPA敲低改变了CRC中的致癌相关基因的表达，DBPA在CRC中通过MAPK信号通路的影响，这可能提供有价值的证据，以进一步研究DBPA表达和CRC开发之间的相关性。

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  • 来源
    《Cell biology international.》 |2016年第12期|共14页
  • 作者

    Liu Ruiting; Wang Guorong; Liu Chang; Qiu Jian; Yan Likun; Li Xiaojun; Wang Xiaoqiang;

  • 作者单位

    Xi An Jiao Tong Univ Shaanxi Prov Peoples Hosp Affiliated Hosp 3 Dept Gen Surg Med Coll Xian;

    Xi An Jiao Tong Univ Shaanxi Prov Peoples Hosp Affiliated Hosp 3 Dept Gen Surg Med Coll Xian;

    Xi An Jiao Tong Univ Affiliated Hosp 1 Coll Med Dept Hepatobiliary Surg Xian 710061 Peoples R;

    Xi An Jiao Tong Univ Shaanxi Prov Peoples Hosp Affiliated Hosp 3 Dept Gen Surg Med Coll Xian;

    Xi An Jiao Tong Univ Shaanxi Prov Peoples Hosp Affiliated Hosp 3 Dept Gen Surg Med Coll Xian;

    Xi An Jiao Tong Univ Shaanxi Prov Peoples Hosp Affiliated Hosp 3 Dept Gen Surg Med Coll Xian;

    Xi An Jiao Tong Univ Shaanxi Prov Peoples Hosp Affiliated Hosp 3 Dept Gen Surg Med Coll Xian;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    colorectal cancer; dpbA; gene expression profiling; microassay; small interfering RNA;

    机译：结直肠癌;DPBA;基因表达谱;微观组播;小干扰RNA;

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