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首页> 外文期刊>Cell biology international. >miR‐370 promotes high glucose‐induced podocyte injuries by inhibiting angiotensin II type 1 receptor‐associated protein
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miR‐370 promotes high glucose‐induced podocyte injuries by inhibiting angiotensin II type 1 receptor‐associated protein

机译：MiR-370通过抑制血管紧张素II型1受体相关蛋白来促进高葡萄糖诱导的致孔细胞损伤

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摘要

Abstract miRNAs expression profiles in podocyte injuries have been reported in different models in vivo and in vitro. In the present study, miR‐370 was elevated in high glucose‐stimulated podocyte, and the post‐transcriptional mechanism of miR‐370 was investigated in high glucose‐induced podocyte injuries. The gene and protein levels were assayed by RT‐qPCR and Western blotting, respectively. Annexin V‐fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining was used to evaluate the apoptosis in high glucose‐stimulated podocyte. The targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. The results demonstrated that over‐activation of miR‐370 was verified in high glucose‐treated podocytes, while miR‐370 repression protected against high glucose‐induced apoptosis, cell membrane, and DNA damage in podocytes. We also found that AGTRAP as a direct target of miR‐370 served as an opposite effect to miR‐370, and overexpression of AGTRAP blocked high glucose‐induced podocytes dysfunction. In conclusions, high glucose‐induced podocytes damage by activating miR‐370 signaling targeted to inhibit the expression of AGTRAP, representing a novel and promising therapeutic target for the treatment of DN.
机译：摘要在体内和体外不同模型中报道了诱饵损伤中的miRNA表达曲线。在本研究中，MiR-370在高葡萄糖刺激的孔细胞中升高，并在高葡萄糖诱导的致孔损伤中研究了miR-370的转录机制。通过RT-QPCR和Western印迹测定基因和蛋白质水平。 Annexin V-荧光素异硫氰酸酯（FITC）/碘化丙酸普（PI）染色用于评估高葡萄糖刺激的孔细胞中的细胞凋亡。通过生物信息学算法预测靶向基因，并通过双荧光素酶报告分析证实。结果表明，MiR-370的过度激活在高葡萄糖处理的致胶质细胞中验证，而MiR-370抑制免受高葡萄糖诱导的细胞凋亡，细胞膜和足细胞的DNA损伤。我们还发现，作为miR-370的直接靶标作为miR-370的直接靶标，并且agtrap的过表达阻断了高葡萄糖诱导的诱变障碍功能障碍。在结论中，通过激活MiR-370信号传导来抑制琼脂表达的高葡萄糖诱导的诱发的诱导的诱导的诱发的诱导的诱导的诱导症状，代表DN治疗的新颖和有前途的治疗靶标。

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  • 来源
    《Cell biology international.》 |2018年第11期|共11页
  • 作者

    Xian Yuxin; Dong Liping; Jia Yong; Lin Yi; Jiao Wenjuan; Wang Yao;

  • 作者单位

    Department of EndocrinologyThe Affiliated Hospital of Qingdao UniversityQingdao 266003 China;

    Department of EndocrinologyQingdao Municipal HospitalNo. 1 Jiaozhou Road Qingdao 266011 China;

    Department of UrologyQingdao Municipal HospitalQingdao 266011 China;

    Department of PediatricsThe Affiliated Hospital of Qingdao UniversityQingdao 266003 China;

    Department of EndocrinologyThe Affiliated Hospital of Qingdao UniversityQingdao 266003 China;

    Department of EndocrinologyQingdao Municipal HospitalNo. 1 Jiaozhou Road Qingdao 266011 China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    AGTRAP; apoptosis; high glucose; miR‐370; podocyte injury;

    机译：agtrap;细胞凋亡;高葡萄糖;mir-370;泛孔细胞损伤;

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