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首页> 外文期刊>Cell biology international. >Spinal cord tissue affects sprouting from aortic fragments in ex vivo co‐culture
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Spinal cord tissue affects sprouting from aortic fragments in ex vivo co‐culture

机译：脊髓组织影响离体培养的主动脉碎片发芽

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Abstract It is a well‐known fact, that there is a close interconnection between vascular and neural structures in both embryonic development and postnatal life. Different models have been employed to dissect the mechanisms of these interactions, ranging from in vitro systems (e.g., co‐culture of neural and endothelial cells) to in vivo imaging of central neural system recovery in laboratory animals after artificially induced trauma. Nevertheless, most of these models have serious limitations. Here, we describe an ex vivo model, representing an organotypic co‐culture of aortic fragments (AF) with longitudinal slices of mouse neonatal spinal cord (SC) or dorsal root ganglia (DRG). The samples were co‐cultured in a medium adapted for SC tissue and lacking any pro‐angiogenic or neurotrophic growth factors. It was found, that cultivation of AFs in the SC injury zone (transversal dissection of a SC slice) resulted in the initiation of active aortic sprouting. Remarkably, the endothelial cells exiting the AFs never invaded the SC tissue, concentrating in a nearby area (negative taxis). In contrast, the DRGs, while also promoting the sprouting, were a target of active endothelial CD31 + cell invasion (positive taxis). Thus, the tissues of both central and peripheral nervous systems have a prominent positive effect on aortic sprouting, while the vector of endothelial cell expansion is strictly nervous‐tissue‐type dependent. The ex vivo AF co‐culture with SC or DRG appeared to be a useful and promising model for a further endeavor into the mechanisms driving the complex interactions between neural and endothelial tissues.
机译：摘要它是一个众所周知的事实，即胚胎发育和后期生命中的血管和神经结构之间存在密切互连。已经采用不同的模型来描述这些相互作用的机制，从体外系统（例如，神经和内皮细胞的共同培养）到人工诱导的创伤后的实验室动物中的中枢神经系统恢复的体内成像。然而，大多数这些模型都有严重的局限性。在这里，我们描述了一个前体内模型，代表主动脉片（AF）的有机型共同培养，具有纵向切片的小鼠新生脊髓（SC）或背根神经节（DRG）。将样品在适于SC组织的培养基中共培养并缺乏任何促血管生成或神经营养生长因子。发现，在SC损伤区（SC切片的横向解剖）中的AFS培养导致活性主动脉萌发的开始。值得注意的是，离开AFS的内皮细胞永远不会侵入SC组织，浓缩在附近的区域（负有关出租车）。相反，DRG，同时还促进发芽，是有源内皮CD31 +细胞侵袭（阳性出租车）的靶标。因此，中枢和外周神经系统的组织对主动脉萌发具有突出的积极作用，而内皮细胞膨胀的载体是严格的神经组织型依赖性。具有SC或DRG的前体内AF共同培养似乎是一种有用和有希望的模型，用于进一步努力进入驱动神经和内皮组织之间复杂相互作用的机制。

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