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首页> 外文期刊>Cell biology international. >Nitric oxide synthase inhibitor L‐NAME affects Arabidopsis Arabidopsis root growth, morphology, and microtubule organization
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Nitric oxide synthase inhibitor L‐NAME affects Arabidopsis Arabidopsis root growth, morphology, and microtubule organization

机译：一氧化氮合酶抑制剂L-NAME影响拟南芥拟南芥根系生长，形态和微管组织

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Abstract The presence of evolutionarily conserved NOS or NOS‐like enzymes in land plants different than those in animals is still unclear, despite their activity has been revealed in cytosol and some organelles. At the same time, the emerging evidence for the importance of L‐arginine‐dependent pathways of NO synthesis in plant cells is still accumulating. The aim of our study was to reveal physiological effects on growth and differentiation processes, and microtubular cytoskeleton organization of the competitive mammalian NO synthase inhibitor Nω‐nitro‐L‐arginine methylester (L‐NAME). Thus, the treatment of Arabidopsis with L‐NAME (50–1?mM) caused dose‐ and time‐dependent inhibition of primary roots growth. Moreover, the morphology of primary roots under the influence of L‐NAME also underwent changes. L‐NAME (100?μM) induced the formation of novel over‐elongated root hairs in shortened elongation zone, while in higher concentrations (500?μM) it caused a slight swelling of epidermal cells in differentiation zone. L‐NAME also provoked microtubule reorganization in epidermal cells of different root growth zones. Thus, L‐NAME at concentrations of 50–1?mM induced cortical microtubules randomization and/or depolymerization in epidermal cells of the root apex, meristem, transition, elongation, and differentiation zones after 2?h of treatment. Disordered microtubules in trichoblasts could initiate the formation of actively elongating root hairs that reveals longitudinal microtubules ensuring their active growth at 24?h of treatment. Therefore, L‐NAME inhibits primary root growth, induces the differentiation processes in roots, reorganizes cortical microtubules in epidermal root cells suggesting the importance of L‐arginine‐dependent pathways of NO synthesis in plants.
机译：摘要在不同于动物中不同的土地植物中进化保守的NOS或NOS样酶的存在仍然尚不清楚，尽管它们的活性在胞质醇和一些细胞器中揭示了它们的活性。与此同时，在植物细胞中没有合成的L-精氨酸依赖性途径的重要性仍然积累。我们的研究目的是揭示对生长和分化过程的生理作用，以及竞争性哺乳动物的微仔细胞骨架组织NO合酶抑制剂Nω-γ-L-精氨酸甲基酯（L-NAME）。因此，用L-名称（50-1×mm）治疗拟南芥（50-1？mm）导致对初级根生长的剂量和时间依赖性抑制。此外，L-NAME影响下的初级根的形态也接受了变化。 L-名称（＆ 100？μm）诱导在缩短的伸长区中形成新型过细胞根毛，而在更高的浓度（500μm）中，它导致分化区中表皮细胞轻微肿胀。 L-NAME还在不同根生长区的表皮细胞中引发微管重组。因此，L-名称浓度为50-1？mm诱导的皮质微管随机化和/或在根目录表皮细胞中随机化和/或解聚，在2〜h后2μmExpex，分类，转变，伸长率和分化区。在三色细胞中的无序的微管可以引发主动伸长的根毛，揭示纵向微管的纵向微管，确保其在治疗中的24μm中的活性生长。因此，L-NAME抑制初级根生长，诱导根系中的分化过程，重新组织表皮根细胞中的皮质微管，表明L-精氨酸依赖性途径在植物中没有合成的重要性。

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