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首页> 外文期刊>Cell and Tissue Research >Effect of thyroid dysfunction on NOS expression in the female rat
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Effect of thyroid dysfunction on NOS expression in the female rat

机译：甲状腺功能障碍对雌性大鼠NOS表达的影响

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摘要

Thyroid hormones (THs) are vital for normal reproductive function and dysregulation of TH impairs follicular development. Although the functions of THs on female reproduction are of great interest, the mechanisms still remain unclear. Many studies have shown that NO plays important roles in female reproduction. In the present study, we investigate the effects of TH dysregulation on nitric oxide synthase types (NOS) expression in rats. Propylthiouracil (PTU) and L-thyroxine were administered to rats to induce hypo- and hyperthyroidism, respectively. Ovarian histology was detected by immunohistochemistry (IHC) and protein or mRNA content was analyzed by Western blotting or RT-PCR, respectively. The results showed that NOS1, NOS2 and NOS3 expressions were detected in the oocyte, granulosa cell and theca cell in all follicular stages, which were up-regulated by eCG treatment. NOS1 protein content was increased in both PTU and L-thyroxine treatments. There were no significant differences in NOS2 levels between the treatment and the control group. However, NOS3 was only increased in the hyperthyroid group. These results were consistent with the IHC staining. The present study provides evidence that TH dysregulation alters NOSs profiles, which suggests that NOSs/nitric oxide (NO) is possibly involved in the regulation of female reproduction.
机译：甲状腺激素（THS）对于正常生殖功能和损害滤饼发育的失调性是至关重要的。虽然THS对女性繁殖的职能具有很大的兴趣，但机制仍然尚不清楚。许多研究表明，没有在女性复制中发挥重要作用。在本研究中，我们研究了Th失衡对大鼠一氧化氮合酶类型（NoS）表达的影响。丙基硫嘧啶（PTU）和L-甲状腺素分别施用于大鼠，分别诱导嗜酸性和甲状腺功能亢进。通过免疫组织化学检测（IHC）检测卵巢组织学，通过蛋白质印迹或RT-PCR分析蛋白质或mRNA含量。结果表明，在所有滤窗阶段中，在卵母细胞，颗粒细胞和Theca细胞中检测到NOS1，NOS2和NOS 3表达，其通过ECG处理上调。在PTU和L-甲状腺素处理中增加了NOS1蛋白质含量。治疗与对照组之间的NOS2水平无显着差异。然而，NOS3仅在甲状腺素基团中增加。这些结果与IHC染色一致。本研究提供了证据表明，Th失去量改变了NOSS型材，这表明诺斯/一氧化物（NO）可能参与了女性繁殖的调节。

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  • 来源
    《Cell and Tissue Research》 |2020年第2期|共10页
  • 作者

    Xu Kaili; Tian Ye; Weng Xuechun; Hu Xusong; Heng Dai; Xia Guoliang; Zhang Cheng;

  • 作者单位

    Capital Normal Univ Coll Life Sci Beijing 100048 Peoples R China;

    Capital Normal Univ Coll Life Sci Beijing 100048 Peoples R China;

    Capital Normal Univ Coll Life Sci Beijing 100048 Peoples R China;

    Capital Normal Univ Coll Life Sci Beijing 100048 Peoples R China;

    Capital Normal Univ Coll Life Sci Beijing 100048 Peoples R China;

    China Agr Univ Coll Biol Sci State Key Lab Agrobiotechnol Beijing 100193 Peoples R China;

    Capital Normal Univ Coll Life Sci Beijing 100048 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Hypothyroid; Hyperthyroid; NOS; Ovary; Rat;

    机译：甲状腺功能亢进;甲状腺功能亢进;NO;卵巢;老鼠;

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