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首页> 外文期刊>Cell and Tissue Research >'Reversed polarization' of Na/K-ATPase-a sign of inverted transport in the human endolymphatic sac: a super-resolution structured illumination microscopy (SR-SIM) study
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'Reversed polarization' of Na/K-ATPase-a sign of inverted transport in the human endolymphatic sac: a super-resolution structured illumination microscopy (SR-SIM) study

机译：Na / K-ATP酶的“反向极化” - 人析瘤囊中倒置输送的标志：超分辨率结构照明显微镜（SR-SIM）研究

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摘要

The human endolymphatic sac (ES) is believed to regulate inner ear fluid homeostasis and to be associated with Meniere's disease (MD). We analyzed the ion transport protein sodium/potassium-ATPase (Na/K-ATPase) and its isoforms in the human ES using super-resolution structured illumination microscopy (SR-SIM). Human vestibular aqueducts were collected during trans-labyrinthine vestibular schwannoma surgery after obtaining ethical permission. Antibodies against various isoforms of Na/K-ATPase and additional solute-transporting proteins, believed to be essential for ion and fluid transport, were used for immunohistochemistry. A population of epithelial cells of the human ES strongly expressed Na/K-ATPase alpha 1, beta 1, and beta 3 subunit isoforms in either the lateral/basolateral or apical plasma membrane domains. The beta 1 isoform was expressed in the lateral/basolateral plasma membranes in mostly large cylindrical cells, while beta 3 and alpha 1 both were expressed with "reversed polarity" in the apical cell membrane in lower epithelial cells. The heterogeneous expression of Na/K-ATPase subunits substantiates earlier notions that the ES is a dynamic structure where epithelial cells show inverted epithelial transport. Dual absorption and secretion processes may regulate and maintain inner ear fluid homeostasis. These findings may shed new light on the etiology of endolymphatic hydrops and MD.
机译：据信人体内淋巴囊（ES）据信调节内耳流体稳态并与Meniere疾病（MD）相关。我们使用超分辨率结构照明显微镜（SR-SIM）分析了人ES中的离子转运蛋白钠/钾 - ATP酶（NA / K-ATP酶）及其同种型。在获得道德许可后，在反式迷住的杂志前庭施瓦膜手术中收集人类前庭渡槽。用于免疫组化，用于免疫为离子和流体运输的各种同种型的抗体和另外的溶质输送蛋白质。在横向/基石或顶端血浆膜结构域中的人ES强烈表达Na / K-ATP酶α1，β1和β3亚单位同种型的群体的上皮细胞。 β1同种型在大多数大的圆柱形电池中在横向/基底间质膜中表达，而β3和α1两者在下皮细胞中的顶端细胞膜中的“反相极性”表示。 Na / K-ATPase亚基的异质表达实质上的观念认为ES是上皮细胞显示倒上皮传输的动态结构。双重吸收和分泌过程可以调节和维持内耳流体稳态。这些发现可以在内淋巴水和MD的病因上揭示新的光线。

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