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首页> 外文期刊>Cell and Tissue Research >Sertoli cell-conditioned medium restores spermatogenesis in azoospermic mouse testis
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Sertoli cell-conditioned medium restores spermatogenesis in azoospermic mouse testis

机译：Sertoli细胞条件培养基恢复含有Zoopospermic小鼠睾丸的精子发生

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摘要

The current study evaluates potential applications of Sertoli cell (SC)-conditioned medium (CM) and explores the effects of the conditioned medium on the spermatogenesis process in azoospermic mice. For this study, 40 adult mice (28-30 g) were divided into 4 experimental groups: (1) control, (2) DMSO 2% (10 mu l), (3) busulfan (40 mg/kg single dose) and (4) busulfan/CM (10 mu l). SCs were isolated from 4-week-old mouse testes. After using anesthetics, 10 mu l of CM was injected over 3-5 min into each testis and subsequently, sperm samples were collected from the tail of the epididymis. Afterward, the animals were euthanized and testis samples were taken for histopathology experiments and RNA extraction in order to examine the expression of c-kit, STRA8 and PCNA genes. The data showed that CM notably increased the total sperm count and the number of testicular cells, such as spermatogonia, primary spermatocytes, round spermatids, SCs and Leydig cells compared with the control, DMSO and busulfan groups. Furthermore, the results showed that expression of c-kit and STRA8 was significantly decreased in the busulfan and busulfan/SC groups at 8 weeks after the last injection (p < 0.001) but no significant difference was found for PCNA compared with the control and DMSO groups (p < 0.05). These findings suggest that the Sertoli cell-conditioned medium may be beneficial as a practical approach for therapeutic strategies in reproductive and regenerative medicine.
机译：目前的研究评估了血清细胞（SC）调节介质（CM）的潜在应用，并探讨了调节培养基对偶氮小鼠的精子发生过程的影响。对于本研究，将40只成人小鼠（28-30g）分为4种实验组：（1）对照，（2）DMSO 2％（10μl），（3）套毛（40 mg / kg单剂量）和（4）Busulfan / cm（10 mu l）。 SCS从4周龄鼠标睾丸中分离出来。在使用麻醉剂后，将10μlcm在3-5分钟内注射到每个睾丸中，随后，从附睾的尾部收集精子样品。之后，将动物进行了安乐死，并考虑了组织病理学实验和RNA提取的睾丸样品，以检查C-kit，STRA8和PCNA基因的表达。数据显示CM显着增加了总精子数量和睾丸细胞的数量，例如精子，初生细胞，圆形精子，SCS和Leydig细胞与对照，DMSO和Busulfan组相比。此外，结果表明，在最后一次注射后8周，在8周后，在伯尔法兰和伯鲁凡/ SC组中表达C-kit和STRA8的表达（P <0.001），但与对照和DMSO相比，PCNA没有发现显着差异组（P <0.05）。这些发现表明，塞敦利细胞条件培养基可能是生殖和再生医学中治疗策略的实用方法。

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  • 来源
    《Cell and Tissue Research》 |2020年第3期|共11页
  • 作者

    Panahi Sakineh; Karamian Amin; Sajadi Ensieh; Aliaghaei Abbas; Nazarian Hamid; Abdi Shabnam; Danyali Samira; Paktinat Shahrokh; Abdollahifar Mohammad-Amin; Farahani Reza Mastery;

  • 作者单位

    Shahid Beheshti Univ Med Sciences Sch Med Dept Biol Anatomical Sciences Tehran Iran Islamic R;

    Shahid Beheshti Univ Med Sciences Sch Med Dept Biol Anatomical Sciences Tehran Iran Islamic R;

    Shahid Beheshti Univ Med Sciences Sch Med Dept Biol Anatomical Sciences Tehran Iran Islamic R;

    Shahid Beheshti Univ Med Sciences Sch Med Dept Biol Anatomical Sciences Tehran Iran Islamic R;

    Shahid Beheshti Univ Med Sciences Sch Med Dept Biol Anatomical Sciences Tehran Iran Islamic R;

    Islamic Azad Univ Fac Med Dept Anatomical Sciences &

    Cognit Neuroscience Tehran Med Sciences;

    Shahid Beheshti Univ Med Sciences Sch Med Dept Physiol Tehran Iran Islamic R;

    Shahid Beheshti Univ Med Sciences Sch Med Dept Biol Anatomical Sciences Tehran Iran Islamic R;

    Shahid Beheshti Univ Med Sciences Sch Med Dept Biol Anatomical Sciences Tehran Iran Islamic R;

    Shahid Beheshti Univ Med Sciences Sch Med Dept Biol Anatomical Sciences Tehran Iran Islamic R;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Sertoli cell-conditioned medium; Spermatogenesis; Azoospermia;

    机译：Sertoli细胞条件培养基;精子发生;偶氮孢子;

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