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首页> 外文期刊>Cell and Tissue Research >Immunohistochemical localization of the NH(2)-terminal and COOH-terminal fragments of dentin sialoprotein in mouse teeth.
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Immunohistochemical localization of the NH(2)-terminal and COOH-terminal fragments of dentin sialoprotein in mouse teeth.

机译：小鼠牙齿牙本质唾液酸蛋白的NH（2）末端和COOH-末端片段的免疫组化定位。

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Dentin sialoprotein (DSP) is a major non-collagenous protein in dentin. Mutation studies in human, along with gene knockout and transgenic experiments in mice, have confirmed the critical role of DSP for dentin formation. Our previous study reported that DSP is processed into fragments in mouse odontoblast-like cells. In order to gain insights into the function of DSP fragments, we further evaluated the expression pattern of DSP in the mouse odontoblast-like cells using immunohistochemistry and western blot assay with antibodies against the NH(2)-terminal and COOH-terminal regions of DSP. Then, the distribution profiles of the DSP NH(2)-terminal and COOH-terminal fragments and osteopontin (OPN) were investigated in mouse teeth at different ages by immunohistochemistry. In the odontoblast-like cells, multiple low molecular weight DSP fragments were detected, suggesting that part of the DSP protein was processed in the odontoblast-like cells. In mouse first lower molars, immunoreactions for anti-DSP-NH(2) antibody were intense in the predentin matrix but weak in mineralized dentin; in contrast, for anti-DSP-COOH antibody, strong immunoreactions were found in mineralized dentin, in particular dentinal tubules but weak in predentin. Therefore, DSP NH(2)-terminal and COOH-terminal fragments from odontoblasts were secreted to different parts of teeth, suggesting that they may play distinct roles in dentinogenesis. Meanwhile, both DSP antibodies showed weak staining in reactionary dentin (RD), whereas osteopontin (OPN) was clearly positive in RD. Therefore, DSP may be less crucial for RD formation than OPN.
机译：牙本霉素唾液蛋白（DSP）是牙本质中的主要非胶原蛋白。人类的突变研究以及小鼠的基因敲除和转基因实验，已经证实了DSP对牙本质形成的关键作用。我们以前的研究报告称DSP在小鼠Ofontoblast样细胞中加工成碎片。为了进入DSP片段的功能，我们进一步使用免疫组织化学和蛋白质印迹测定与抗抗体的NH（2）末端和DSP的CoOH末端区域的抗体评估DSP在小鼠Odontoblast样细胞中的表达模式。 。然后，通过免疫组织化学在不同年龄的小鼠牙齿中研究了DSP NH（2）末端和CoOH-末端片段和骨桥蛋白（OPN）的分布曲线。在异常子细胞样细胞中，检测多个低分子量DSP片段，表明在异常状细胞中加工了DSP蛋白的一部分。在小鼠第一较低的摩尔方中，预测抗DSP-NH（2）抗体的免疫反应在预测基质中是强烈的，但在矿化牙本质中弱;相反，对于抗DSP-CoOH抗体，在矿化牙本质中发现强免疫凋亡，特别是牙本质小管，但预测中弱。因此，来自Odontoblasts的DSP NH（2）末端和CoOH-末端片段分泌到不同部分的牙齿，表明它们可能在牙本发生中起不同的作用。同时，DSP抗体都显示出反动牙本质（RD）的染色弱染色，而骨桥蛋白（OPN）在RD中明显呈阳性。因此，DSP比OPN的RD形成不均匀。

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