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首页> 外文期刊>Cell and Tissue Research >Characterisation of vitamin D-related molecules and calcium-sensing receptor in human Fallopian tube during the menstrual cycle and in ectopic pregnancy
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Characterisation of vitamin D-related molecules and calcium-sensing receptor in human Fallopian tube during the menstrual cycle and in ectopic pregnancy

机译：在月经周期和异位妊娠中人输卵管中维生素D相关分子和钙传感受体的表征

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This is a prospective observational study that measures the expression of vitamin D (VD) metabolising and signalling molecules and Ca2+ sensing receptor (CaSR) in human Fallopian tube (FT) during the menstrual cycle and ectopic pregnancy (EP). Fresh FTs were obtained during total abdominal hysterectomy at the follicular (n = 16) and midluteal (n = 16) phases. Specimens from remote and implantation sites as well as trophoblastic tissues were also freshly collected from each FT with EP (n = 10). All women had normal serum VD and ionised Ca2+. The expression of VD synthesising (CYP27B1) and catalysing (CYP24A1) enzymes, binding protein (VDBP), receptor (VDR), retinoid X receptor (RXR) and CaSR was measured by immunohistochemistry and quantitative RT-PCR. All molecules, except VDBP, were significantly increased (P < 0.05) in midluteal compared with follicular samples. Remote EP sites showed significantly (P < 0.05) lower expression of CYP27B1, CYP24A1, VDR and RXR and a higher expression of VDBP and CaSR (P < 0.05) compared with midluteal samples. Significant differences were observed by immunohistochemistry between implantation and remote sites from EP for all molecules, which were also localised in the trophoblastic tissues. In conclusion, VD and calcium are under cycle-dependent regulations within human FT and they appear to play a role in tubal biology through paracrine/autocrine mode of signalling. Furthermore, EP was associated with alterations in the expression of all the studied molecules by the tubal epithelium. Further studies are needed to explore the roles of VD in tubal biology and pathogenesis of EP.
机译：这是一种预期的观察性研究，可在月经周期和异位妊娠（EP）期间测量人类输卵管（FT）中的维生素D（VD）代谢和信号分子和CA2 +传感受体（CASR）的表达。在滤泡（n = 16）和中脑（n = 16）相的总腹腔子宫切除术期间获得新鲜FTS。来自远程和植入部位的标本以及滋养细胞组织也从每个FT与EP（n = 10）新鲜收集。所有女性都有正常的血清VD和电离CA2 +。通过免疫组织化学和定量RT-PCR测量VD合成（CYP27B1）和催化（CYP27B1）和催化剂（CYP2A1），结合蛋白（VDBP），受体（RXR）和CASR的表达。除滤泡样品相比，除VDBP之外的所有分子在中肠中显着增加（P <0.05）。与中肺样品相比，远程EP位点显着（P <0.05）CYP27B1，CYP24A1，VDR和RXR的表达和较高的VDBP和CASR的表达（P <0.05）。通过对所有分子的EP的植入和远程位点之间的免疫组织化学观察显着差异，其在所有分子中也局部化在滋养细胞组织中。总之，VD和钙在人FT中依赖于循环依赖的规定，它们似乎通过帕拉卡碱/自分泌模式发挥输卵管生物学中的作用。此外，EP与输卵管上皮的所有研究分子表达的改变有关。需要进一步的研究来探讨VD在EP输卵管生物学和发病机制中的作用。

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