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首页> 外文期刊>Cell and Tissue Research >Quiescent adult stem cells in murine teeth are regulated by Shh signaling
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Quiescent adult stem cells in murine teeth are regulated by Shh signaling

机译：鼠齿的静态成体干细胞由SHH信号传导调节

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摘要

The mechanisms regulating the maintenance of quiescent adult stem cells in teeth remain to be fully elucidated. Our aim is to clarify the relationship between BrdU label-retaining cells (LRCs) and sonic hedgehog (Shh) signaling in murine teeth. After prenatal BrdU labeling, mouse pups were analyzed during postnatal day 1 (P1) to week 5 (P5W). Paraffin sections were processed for immunohistochemistry for BrdU, Sox2, Gli1, Shh, Patched1 (Ptch1) and Ki67 and for in situ hybridization for Shh and Ptch1. Dense LRCs, Gli1-(+) cells and Ptch1-(+) cells were co-localized in the outer enamel epithelium of the apical bud and apical dental papilla of incisors. In developing molars, dense LRCs were numerous at P1 but then decreased in number over the course of odontogenesis and were maintained in the center of pulp tissue. Gli1-(+) cells were maintained in the pulp horn during the examined stages, while they increased in number and were maintained in the center of pulp tissue during P2-5W. Ptch1-(+) cells were localized in the pulp horn at P1 and increased in number in the center of the pulp after P3W. Shh mRNA was first expressed in the enamel epithelium and then shifted to odontoblasts and other pulp cells. Shh protein was distributed in the epithelial and mesenchymal tissues of incisors and molars. These findings suggest that quiescent dental stem cells are regulated by Shh signaling, and that Shh signaling plays a crucial role in the differentiation and integrity of odontoblasts during epithelial-mesenchymal interactions and dentinogenesis.
机译：调节牙齿中静态成年干细胞维持的机制仍然被完全阐明。我们的目的是阐明Brdu标签静置细胞（LRC）和Sonic Hedgehog（Shh）信号传导的关系。在产前Brdu标记后，在后期第1天（P1）至第5周（P5W）期间分析小鼠幼崽。将石蜡切片用于Brdu，Sox2，Gli1，ShH，浸渍的1（PTCH1）和Ki67的免疫组织化学以及用于SHH和PTCH的原位杂交。致密LRC，Gli1 - （+）细胞和PTCH1 - （+）细胞在外侧牙釉质上皮的外牙釉质上皮和切口的顶端牙乳乳头的外牙釉质上皮。在开发臼齿中，致密的LRC在P1下无数，但随后在牙核发生过程中的数量减少，并保持在纸浆组织的中心。在检查的阶段期间，Gli1 - （+）细胞在纸浆角中保持在纸浆喇叭中，而它们在数量上增加并且在P2-5W期间保持在纸浆组织的中心。 PTCH1 - （+）细胞在P1处的纸浆喇叭定位，并在P3W后纸浆中心的数量增加。首先在牙釉质上皮中表达SHH mRNA，然后转移到Odontoblasts和其他纸浆细胞中。 SHH蛋白分布在切牙和臼齿的上皮和间充质组织中。这些发现表明，静态牙科干细胞由SHH信号调节，并且SHH信号传导在上皮 - 间充质相互作用和牙本发生期间在Odontobrasts的分化和完整性中起着至关重要的作用。

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