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首页> 外文期刊>Cell and Tissue Research >Time-of-day-dependent expression of purinergic receptors in mouse suprachiasmatic nucleus
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Time-of-day-dependent expression of purinergic receptors in mouse suprachiasmatic nucleus

机译：小鼠的嘌呤能受体依赖于小鼠的嘌呤能受体的时间表达

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摘要

Purinergic P2X and P2Y receptors are involved in mediating intercellular signalling via purines such as adenosine triphosphate (ATP). P2X and P2Y receptors have been implicated in numerous body functions including learning, memory and sleep. All of these body functions show time-of-day-dependent variations controlled by the master circadian oscillator located in the suprachiasmatic nucleus (SCN). Evidence exists for a role of purinergic signalling in intercellular coupling within SCN. However, few studies have been performed on the expression of purinergic receptors in SCN. Therefore, we analyse the expression of seven P2X (P2X1-7) and eight P2Y (P2Y1-2, 4, 6, 11-14) receptors in mouse SCN and address their time-of-day-dependent variation by using immunohistochemistry and real-time polymerase chain reaction. At the early light phase, P2X and P2Y receptors show a low to moderate, homogenously distributed immunoreaction throughout SCN. P2Y13 reveals strong immunoreaction in fibres within the core region of SCN. From the fifteen analysed P2 receptors, seven exhibit a time-of-day-dependent variation in SCN. P2X1 immunoreaction is very low in the early light phase with a minor increase at the end of the dark phase. P2X4 immunoreaction strongly increases during the dark phase in soma cells in the core region and in a dense network of fibres in the shell region of SCN. P2X3 immunoreaction is moderately elevated during the dark phase. Conversely, immunoreaction for P2Y2, P2Y12 and P2Y14 moderately increases at the early light phase and P2Y6 immunoreaction displays a moderate increase at the mid-light phase. Thus, this study demonstrates a time-of-day-dependent variation of P2 receptors in mouse SCN.
机译：嘌呤能P2X和P2Y受体涉及通过嘌呤如腺苷三磷酸（ATP）介导细胞间信号传导。 P2X和P2Y受体在包括学习，记忆和睡眠的许多身体功能中涉及。所有这些身体功能都显示了由位于Suprachiasmatic核（SCN）中的主昼夜昼夜振荡器控制的一致依赖变化。存在嘌呤能信号传导在SCN内细胞间偶联中的作用的证据。然而，已经对SCN中的嘌呤能受体表达进行了少数研究。因此，我们分析小鼠SCN中七个P2X（P2X1-7）和八个P2Y（P2Y1-2,4,6,114,2114）的表达，并通过使用免疫组化和真实来解决它们依赖于时间的变化 - 达特聚合酶链反应。在早期光相时，P2X和P2Y受体显示出在整个SCN的低至中等的，均质分布的免疫反应。 P2Y13揭示了SCN核心区域内的纤维中的强烈免疫反应。从十五分析的P2受体中，七个表现出SCN的一天依赖性变化。 P2X1免疫反应在早期光相中非常低，在黑暗相结束时轻微增加。 P2X4免疫反应在核心区域的SOMA细胞中的黑暗相期间强烈增加，并且在SCN的壳区域中的纤维网络中。在黑暗相期间，P2X3免疫入射适度升高。相反，在早期光相时，P2Y2，P2Y12和P2Y14的免疫反应在早期的光相时中适度地增加，P2Y6免疫反应在中间光相时显示中等增加。因此，该研究证明了小鼠SCN中P2受体的一时间依赖性变化。

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