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首页> 外文期刊>Cell and Tissue Research >Development of an in vitro model to study uterine functions and early implantation using rat uterine explants
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Development of an in vitro model to study uterine functions and early implantation using rat uterine explants

机译：使用大鼠子宫外植体研究子宫功能和早期植入的体外模型

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This study was conducted to develop an in vitro model using rat uterine explants to explore complex uterine functions. Rat uterine explants (1-2 mm) were isolated, cultured and further characterized. Steroid hormone treatment of cultured explants showed that both Muc1 and Pr were significantly up-regulated (P < 0.05) by E2. Areg was significantly up-regulated (P < 0.05) by P4 and Igfbp1 was significantly up-regulated (P < 0.05) by the combination of E2 and P4, although, in rat, Igfbp1 is E2-dependent. In vitro decidualization of cultured explants was induced and two potential markers of decidualization, Prl8a2 and Bmp2, were examined. Real-time quantitative PCR data revealed that both Prl8a2 and Bmp2 were significantly up-regulated (P < 0.05) in MPA- and db-cAMP-treated explants compared to the control group of explants. Then, an individual hatched blastocyst and cultured explant was placed in a 96-well (round-bottom U-shaped) plate. Co-culture results showed that stable attachments were observed after 48 h, where embryos were stably attached to the explants and could not be dislodged after mild shaking and/or pipetting. The rates of attachment of embryos to the explants were increased significantly in the P4-treated group (63.6%) compared to the control group (35.5%), after steroid hormone treatment. The rates of attachment were reduced significantly in the E2-treated group (0.0%), where no stable attachments were observed. Despite the necessity of comprehensive investigation, our results suggest that the cultured rat uterine explants can be a useful in vitro model to study uterine functions and early implantation.
机译：进行该研究以使用大鼠子宫外植体开发体外模型，以探索复杂的子宫功能。分离大鼠子宫外植体（1-2毫米），培养和进一步表征。培养的外植体的类固醇激素治疗表明，MUC1和PR两者均明显上调（P <0.05）。通过P4和IGFBP1显着上调（P <0.05），通过E2和P4的组合显着上调（P <0.05），但在大鼠中，IGFBP1是E2依赖性的。诱导培养的外植体的体外蜕皮化，研究了两种潜在的蜕皮病，PRL8A2和BMP2标记。实时定量PCR数据显示，与对照组的外植体相比，PRL8A2和BMP2在MPA和DB-CAMP处理的外植体中显着上调（P <0.05）。然后，将个体阴影胚泡和培养的外植化置于96孔（圆底U形）板中。共培养结果表明，48小时后观察到稳定的附着，其中胚胎稳定地连接到外植体，在轻度摇动和/或移液后不能脱落。与对照组（35.5％）在类固醇激素处理后，P4处理组（63.6％）在P4治疗组（63.6％）中，胚胎对外植体的附着率显着增加。在E2处理组（0.0％）中，附着的速率显着降低，其中观察到没有稳定的附着物。尽管必须进行全面调查，我们的结果表明，培养的大鼠子宫外植体可以是研究子宫功能和早期植入的有用的体外模型。

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